Antibodies

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Primärantikörper

Katalog-Nr. Wirt Iso. Klon Präs. Reakt. Anwendungen  

6xHistidine Epitope Tag (HHHHHH) Antikörper

Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 µg/ml was used to detect 1.0 µg of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye(R) 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 30 min at room temperature. LICORs Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. Rabbit HRP C, E, WB
0.1 mg / 480,00 €
  OriGene Technologies GmbH

6xHistidine Epitope Tag (HHHHHH) Antikörper

Western blot analysis of His-Ubiquitin recombinant protein probed with MOUSE ANTI HISTIDINE TAG:HRP (SM1693HRP). Mouse IgG1 AD1.1.10 HRP E, WB
0.1 mg / 550,00 €
  OriGene Technologies GmbH

Albumin Antikörper

  Rabbit polyclonal Human Serum Albumin antibody Peroxidase Conjugated  
WB of human serum albumin. Lane 1: 1:400 of FBS. Lane 2: 1:1600 of FBS. Lane 3: 1:400 of normal rabbit serum. Lane 4: 1:1600 of normal rabbit serum. Lane 5: 1:400 of normal human serum. Lane 6: 1:1600 of normal human serum. Lane 7: 1:10 of human serum albumin (10mg/mL). Lane 8: 1:40 of human serum albumin (10mg/mL). Lane 9: molecular weight. Right Blot: Secondary only incubated for control. Primary antibody: human serum albumin at 1:400 . Rabbit HRP Hu E, WB
1 mg / 1.345,00 €
  OriGene Technologies, Inc.

Aldolase A / ALDOA Antikörper

IgG purified antibody to rabbit muscle aldolase (R1054, R1054P and R1054HRP) was used at a 1/1000 dilution to detect human aldolase by western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4° C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kDa consistent with human aldolase. Goat HRP Hu, Rb E, IP, WB
0.1 mg / 515,00 €
  OriGene Technologies GmbH

Beta-2-microglobulin Antikörper

Western Blotting analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-Human β2-microglobulin antibody (B2M-01). 
Lane 1: RAJI Human Burkitt lymphoma cell line.
Lane 2: EL4 Mouse lymphoblastic lymphoma cell line. 
Lane 3: U937 Human histiocytic lymphoma cell line. Mouse IgG2a B2M-01 HRP Hu E, WB
0.1 mg / 280,00 €
  OriGene Technologies GmbH

Bilirubin oxidase Antikörper

Western blot of goat anti Bilirubin oxidase antibody peroxidase conjugated Cat.-No. R1066HRP. Lane 1: Bilirubin oxidase (Myrothecium verrucaria). Load: 50 ng per lane. Primary antibody: Goat anti-Bilirubin oxidase (Myrothecium verrucaria) antibody peroxidase conjugated at 1/1,000 overnight at 4°C. Secondary antibody: n/a. Block: MB-070 for 30 minutes at RT. Predicted/Observed size: 64 kDa, 60 kDa for Bilirubin oxidase. Other band(s): Bilirubin oxidase splice variants and isoforms. Goat HRP Myrothecium E, IP, WB
0.1 mg / 515,00 €
  OriGene Technologies GmbH

Carboxypeptidase B Antikörper

Western blot of peroxidase conjugated rabbit anti-Carboxypeptidase B antibody Cat.-No. R1071HRP . Lane 1: Carboxypeptidase B. Load: 50 ng per lane. Primary antibody: Peroxidase conjugated rabbit anti-Carboxypeptidase B antibody at 1/1,000 overnight at 4°C. Secondary antibody: n/a. Blocking buffer for 30 minutes at RT. Predicted/Observed size: 47 kDa, observed at 35 kDa for Carboxypeptidase B. Expected molecular weight for processed protein is ~34.7 kDa. Rabbit IgG HRP Por E, WB
0.1 mg / 515,00 €
  OriGene Technologies GmbH

CAS9 Antikörper

  Anti-CAS9 Antibody  
Western blot was performed on protein extracts from HeLa cells transfected with a FLAG-tagged Cas9 using the Cas9 antibody. The antibody was used at different dilutions. The marker is shown on the left, the position of the FLAG-tagged Cas9 protein is indicated on the right. Mouse IgG1 7A9 HRP S. pyogenes WB
0.5 ml / 620,00 €
  OriGene Technologies, Inc.

GFP Antikörper

Western Blot of Anti-GFP (MOUSE) Monoclonal Antibody Peroxidase Conjugate. Lane 1: 50ng of GFP. Lane 2: none. Primary antibody: none. Secondary antibody: Anti-GFP (Mouse) Monoclonal Antibody Peroxidase Conjugate (Cat.-No R1461HRP) secondary antibody was used at 1:1000 for 45 min at RT. Block: 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20°C. Predicted/Observed size: 28 kDa for GFP. Other band(s): none. Mouse IgG2a 9F9.F9 HRP A. victoria C, E, WB
1 mg / 855,00 €
  OriGene Technologies GmbH

Glucose Oxidase Antikörper

Western Blot of Rabbit anti-Glucose Oxidase Antibody Peroxidase Conjugated. Lane 1: Glucose Oxidase. Load: 50 ng per lane. Primary antibody: Rabbit anti-Glucose Oxidase Antibody Peroxidase Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: n/a. Block: MB-070 for 30 min at RT. Predicted/Observed size: 66 kDa, 66 kDa for Glucose Oxidase. Detected using FEMTOMAX-110 substrate. Rabbit HRP Aspergillus C, E, WB
0.1 mg / 515,00 €
  OriGene Technologies GmbH

Hexokinase-1 Antikörper

Western blot of rabbit anti-Hexokinase antibody peroxidase conjugated Cat.-No. R1093HRP: Lane 1: Hexokinase. Load: 50 ng per lane. Primary antibody: Rabbit anti-Hexokinase antibody peroxidase conjugated at 1/1,000 overnight at 4°C. Secondary antibody: n/a, blocking buffer for 30 min at RT. Predicted/Observed size: 54 kDa, 54 kDa for Hexokinase. Rabbit HRP Ye E, WB
0.1 mg / 515,00 €
  OriGene Technologies GmbH

Interleukin-27 / IL27A Antikörper

  Rabbit polyclonal IL-27/p28 antibody Peroxidase Conjugated  
Recombinant mouse IL27/p28 was loaded at 0.25 µg. The blot was blocked with 1% BSA in TBST for 30 min at RT. Blot was incubated with HRP rabbit anti-Mouse IL-27/p28 in 1% BSA/TBST at 1:5,000 for 30 min at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP. Rabbit IgG HRP Ms E, WB
0.1 mg / 515,00 €
  OriGene Technologies, Inc.

Interleukin-32 / IL32 A Antikörper

  Rabbit polyclonal IL-32A antibody Peroxidase Conjugated  
Western blot using HRP conjugated anti-Human IL-32A antibody shows detection of a band ~19 kDa in size corresponding to recombinant human IL-32A. The identity of the higher molecular weight band is unknown. Molecular weight markers are shown (left). After transfer, the membrane was blocked with 3% BSA in TBS followed by reaction with antibody at a 1:5,000 dilution for 30 min at room temperature. Detection occurred using TMB substrate. Rabbit IgG HRP Hu C, E, WB
0.1 mg / 515,00 €
  OriGene Technologies, Inc.

Interleukin-33 (IL33) Antikörper

  Rabbit polyclonal IL-33 antibody Peroxidase Conjugated  
Western blot using HRP conjugated anti-Human IL-33 antibody shows detection of a band ~18 kDa in size corresponding to recombinant human IL-33. The identity of the higher molecular weight band is unknown. Molecular weight markers are shown (left). After transfer, the membrane was blocked with 3% BSA in TBS followed by reaction with antibody at a 1:5,000 dilution for 30 min at room temperature. Detection occurred using TMB substrate. Rabbit IgG HRP Hu E, WB
0.1 mg / 515,00 €
  OriGene Technologies, Inc.

L-asparaginase 1 Antikörper

Western blot of peroxidase conjugated rabbit anti- L-asparaginase antibody Cat.-No. R1059HRP. Lane 1: L-asparaginase. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking buffer for 30 min RT. Predicted/observed size: 30 kDa for L-asparaginase. Other band(s): L-asparaginase splice variants and isoforms. Rabbit HRP E. coli E, WB
0.1 mg / 515,00 €
  OriGene Technologies GmbH

Ovalbumin Antikörper

Western blot of rabbit anti-Ovalbumin peroxidase conjugated antibody Cat.-No R1101HRP. Lane 1: Ovalbumin (hen egg). Load: 50 ng per lane. Primary antibody: Rabbit anti-Ovalbumin peroxidase conjugated antibody at 1/1,000 overnight at 4°C. Secondary antibody: none. Blocking buffer for 30 min at RT. Predicted/observed size: 43 kDa for Ovalbumin. Other band(s): Ovalbumin splice variants and isoforms. Rabbit HRP Chk C, E, IP, WB
0.1 mg / 515,00 €
  OriGene Technologies GmbH

RFP-Tag (Ads. to Hu, Ms, Rt Serum Proteins) Antikörper

Western blot of RFP recombinant protein detected with anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 μg of HeLa cell extract. Lane 2 shows a single band detected in 10 μg of a HeLa lysate containing RFP recombinant protein. Anti-RFP detects a 27 kDa band corresponding to the epitope tag RFP. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody diluted 1:2,500 for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye(TM)800 conjugated Goat-a-Rabbit IgG [H&L]. IRDye(TM)800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit IgG HRP C, E, WB
0.1 mg / 515,00 €
  OriGene Technologies GmbH

RNASE1 Antikörper

Western blot of rabbit anti-Ribonuclease A antibody peroxidase conjugated Cat.-No. R1109HRP. Lane 1: Ribonuclease A (bovine pancreas). Load: 50 ng per lane. Primary antibody: Rabbit anti-Ribonuclease A antibody peroxidase conjugated at 1/1,000 overnight at 4°C. Secondary antibody: n/a. Blocking buffer for 30 min at RT. Predicted/Observed size: 16.5 kDa, 16.5 kDa for Ribonuclease A. Rabbit HRP Bov E, IP, WB
0.1 mg / 515,00 €
  OriGene Technologies GmbH

Superoxide Dismutase 1 / SOD1 Antikörper

Western blot of HRP conjugated Superoxide Dismutase antibody Cat.-No. R1111HRP). Lane 1: Superoxide Dismutase load: 50 ng per lane. Primary antibody: HRP conjugated Superoxide Dismutase antibody at 1/1,000 overnight at 4°C. Secondary antibody: none; HRP substrate. Predicted/observed size: 15.6 kDa, 15.6 kDa. Rabbit IgG HRP Bov E, WB
0.1 ml / 515,00 €
  OriGene Technologies GmbH

Trypsinogen Antikörper

Western blot of peroxidase conjugated rabbit anti-Trypsinogen primary antibody Cat.-No. R1116HRP. Lane 1: Trypsinogen.  Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rabbit secondary antibody at 1/1,000 for 60 min at RT. Blocking buffer for 30 min at RT. Predicted/observed size: 24 kDa, 24 kDa for Trypsinogen. Other band(s): None. Rabbit HRP Bov E, WB
0.1 mg / 515,00 €
  OriGene Technologies GmbH

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