Antibodies

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Primärantikörper

Katalog-Nr. Wirt Iso. Klon Präs. Reakt. Anwendungen  

14-3-3 protein sigma / SFN Antikörper

  Rabbit Polyclonal 14-3-3 sigma/Stratifin Antibody  
Western Blot: 14-3-3 sigma/Stratifin Antibody [TA337133] - Analysis of 14-3-3 sigma (169 amino acid isoform) using 14-3-3 sigma antibody.  Human brain tissue in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain tissue p Rabbit Purified Amph, Bov, Can, Eq, Hu, Op P, WB
0.1 mg / 395,00 €
  OriGene Technologies, Inc.

Glypican-1 / GPC1 Antikörper

  Rabbit polyclonal Glypican-1 antibody  
WB of Rabbit anti-Glypican-1 antibody. Lane 1: untransfected 293T cell lysate. Lane 2: 293T whole cell lysate. Load: 50ug per lane. Primary antibody: Glypican-1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Gt-a-Rabbit IgG diluted 1:5,000 at 4° C. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 61kDa, 61kDa for Glypican-1. Other band(s): 110kDa is likely due to the presence of the Fc-tag. Minor bands may represent post translational modifications of glypican-1. Rabbit IgG Aff - Purified Can, Hu, Macaque, Ms, Op, Rt E, WB
0.1 mg / 515,00 €
  OriGene Technologies, Inc.

LC3 Antikörper

  Rabbit Polyclonal LC3/MAP1LC3A Antibody  
Western Blot: LC3 Antibody [TA336967] - Lysate from human brain in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain probed with ATG8 antibody at 1 ug/ml. Rabbit IgG Purified Bov, Hu, Ms, Op, Prim, Rt P, WB
0.1 mg / 395,00 €
  OriGene Technologies, Inc.

MYB Antikörper

Western blot using affinity purified anti-C-Myb antibody in Cos7 cell lysates: Lane 1: vector; Lane 2: transfected with c-myb, no treatment; Lane 3: transfected with c-Myb and heat stress-treated (incubation at 42οC for 30 minutes) to induce c-Myb modification. Lane 3 shows detection of bands at 75, 98, and 125kDa corresponding to overexpressed c-Myb (arrows): wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively. Nitrocellulose 0.45µm membrane was blocked for 1 hr at RT in 10% BLOTTO in PBS. The primary antibody was used at 1:500. Rabbit IgG Aff - Purified Bonobo, Chimp, Eq, Gorilla, Hu, Mky, Ms, Op, Orang-Utan, Por, Rt E, WB
0.1 mg / 515,00 €
  OriGene Technologies GmbH

MYB Antikörper

Western blot using affinity purified anti-C-Myb antibody in Cos7 cell lysates: Lane 1: vector; Lane 2: transfected with c-myb, no treatment; Lane 3: transfected with c-Myb and heat stress-treated (incubation at 42οC for 30 minutes) to induce c-Myb modification. Lane 3 shows detection of bands at 75, 98, and 125kDa corresponding to overexpressed c-Myb (arrows): wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively. Nitrocellulose 0.45µm membrane was blocked for 1 hr at RT in 10% BLOTTO in PBS. The primary antibody was used at 1:500. Rabbit IgG Aff - Purified Bonobo, Chimp, Eq, Gorilla, Hu, Mky, Ms, Op, Orang-Utan, Por, Rt E, WB
25 µl / 190,00 €
  OriGene Technologies GmbH

RBL2 / p130 (SPA310) Antikörper

  Rabbit polyclonal pRb2 p130 antibody  
WB using Anti-Spa310 antibody shows detection of endogenous pRb2/p130 protein in whole LNCaP cell extracts. The band at ~130 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of pRb2/p130. The membrane was blocked overnight with a milk buffer at 4?. Primary antibody was diluted 1:500 and reacted with the membrane overnight at 4?. ECL was used for detection. Rabbit IgG Aff - Purified Bov, Can, Chimp, Eq, Hu, Op, Orang-Utan, Rt E, IP, WB
0.1 mg / 515,00 €
  OriGene Technologies, Inc.

RPS6KA1 pSer732 Antikörper

Western blot using affinity purified antip90 RSK1 pS732 antibody shows detection of a band ~90 kDa in size corresponding to phosphorylated p90 RSK1 (arrowhead) in EGF stimulated (lane 2) HEK293T cell lysates prepared from cells grown in the absence of serum for 12 h. No staining is observed in similarly prepared lysates derived from unstimulated (control) cells (lane 1). After transfer, the membrane was blocked overnight followed by reaction with the primary antibody at a 1:1,000 dilution. Detection occurred using a peroxidase conjugated secondary antibody and ECL. Rabbit IgG Aff - Purified Bov, Can, Chk, Eq, Hu, Ms, Op, Rt E, WB
0.1 mg / 515,00 €
  OriGene Technologies GmbH

RPS6KA1 pSer732 Antikörper

Western blot using affinity purified antip90 RSK1 pS732 antibody shows detection of a band ~90 kDa in size corresponding to phosphorylated p90 RSK1 (arrowhead) in EGF stimulated (lane 2) HEK293T cell lysates prepared from cells grown in the absence of serum for 12 h. No staining is observed in similarly prepared lysates derived from unstimulated (control) cells (lane 1). After transfer, the membrane was blocked overnight followed by reaction with the primary antibody at a 1:1,000 dilution. Detection occurred using a peroxidase conjugated secondary antibody and ECL. Rabbit IgG Aff - Purified Bov, Can, Chk, Eq, Hu, Ms, Op, Rt E, WB
25 µl / 190,00 €
  OriGene Technologies GmbH

SLC9A3 / NHE3 Antikörper

  Mouse Monoclonal NHE3 [p Ser552] Antibody (14D5)  
Western Blot:  Sodium / Hydrogen Exchanger 3 [phospho Ser552] Antibody (14D5) - Detection of NHE3 [S552] in human kidney lysate.. Mouse 14D5 Purified Hu, Ms, Op, Rb, Rt C, E, ICC/IF, IP, WB
0.1 ml / 515,00 €
  OriGene Technologies, Inc.

SMAD1 Antikörper

  Rabbit polyclonal anti-SMAD1 antibody  
WB using Anti-SMAD1 antibody shows detection of endogenous SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) and keratinocyte (HaCaT, lanes 5-8) derived cell lines treated with PBS, BMP2, EGF, or NaCl for 1 h at 37? before harvest. Each lane contains approximately 15 µg of lysate. Primary antibody was used at 1:500. Anti-beta actin staining was used as a loading control. The membrane was washed and reacted with a 1:3,000 dilution HRP-conjugated a-Rabbit IgG. Rabbit IgG Aff - Purified Bonobo, Can, Cattle, Chimp, Chk, Dros, Duckbill platypus, Eq, Gorilla, Hu, Macaque, Ms, Op, Orang-Utan, Por, Rt, Salmon, Sh E, WB
0.1 mg / 515,00 €
  OriGene Technologies, Inc.

SMAD1 pSer206 Antikörper

  Rabbit polyclonal SMAD1 phospho S206 antibody  
WB using Anti-SMAD1 pS206 antibody shows detection of endogenous phosphorylated SMAD1 in whole cell lysates from HEPG2 (lanes 1-4) derived cell lines treated with PBS, BMP2 (5ng/mL), EGF (1ng/mL), or NaCl for 1h at 37? before harvest. Primary antibody was used at 1:500 and pre-incubated before reacting with blot as follows: top row - with PBS,middle row - with the immunizing phosphorylated peptide and bottom row - with control or non-phosphorylated peptide. HRP-conjugated-Rabbit IgG was 1:3,000. Rabbit IgG Aff - Purified Bonobo, Can, Cattle, Chimp, Chk, Dros, Duckbill platypus, Eq, Gorilla, Hu, Macaque, Ms, Op, Orang-Utan, Por, Rt, Salmon, Sh E, WB
0.1 mg / 515,00 €
  OriGene Technologies, Inc.

SOX17 Antikörper

  Rabbit Polyclonal SOX17 Antibody  
Western Blot: SOX17 Antibody [TA337006] - analysis of SOX17 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide using SOX17 antibody at 1 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solut Rabbit Purified Hu, Ms, Op, Prim, Rt, Ze P, WB
0.1 mg / 450,00 €
  OriGene Technologies, Inc.

UBE2J2 Antikörper

  Rabbit polyclonal Ubiquitin-Conjugating Enzyme E2 J2 antibody  
Western blot using affinity purified anti-Ube2j2 antibody shows detection of Ube2j2 in 293 cells over-expressing Myc-Ube2j2 (Lane 1). Lane 2 contains lysate from mock-transfected 293 cells. Personal Communication, A. Weissman & T. Shang, CCR-NCI, Frederick, MD Rabbit IgG Aff - Purified Can, Cattle, Eq, Hu, Macaque, Ms, Op, Platypus, Rt, Salmon, Xen E, IP, WB
0.1 mg / 515,00 €
  OriGene Technologies, Inc.

WNT1 Antikörper

  Rabbit polyclonal anti-Wnt1 antibody  
WB using Anti-Wnt1 antibody shows detection of endogenous Wnt1 in human-derived MCF7 cell lysate (lane 1) and mouse-derived 3T3 cell lysate (lane 2). The band at ~41 kDa, indicated by the arrowhead, corresponds to Wnt1. After transfer, the membrane was blocked with 5% BLOTTO. Primary antibody was used at a 1:1,400 dilution in PBS containing 1% BLOTTO. The specificity of the antibody was confirmed by peptide competition which completely blocked reaction of the antibody with Wnt1 (data not shown). Rabbit IgG Aff - Purified Bov, Can, Hu, Mky, Ms, Op, Rt E, WB
0.1 mg / 515,00 €
  OriGene Technologies, Inc.

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