Antibodies

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Primärantikörper

Katalog-Nr. Wirt Iso. Klon Präs. Reakt. Anwendungen  

Ah receptor / AhR Antikörper

  Goat Polyclonal AHR Antibody  
Western Blot: Aryl hydrocarbon Receptor Antibody [TA336259] - U937 cell nuclear protein. Image from verified customer review. Goat Purified Hu, Ms, Rt EMSA, WB
1 ml / 395,00 €
  OriGene Technologies, Inc.

CREB1 (N-term) Antikörper

R1188 CREB antibody is shown to detect CREB-1 present in Raji B cell nuclear extract lysates. Detection occurs using a 1/1,000 dilution of antibody followed by 1/5,000 dilution of HRP Goat-anti Rabbit IgG with visualization via ECL. Film exposure approximately 1'. Other detection systems will yield similar results. Rabbit Liquid Hu, Ms, Rt E, EMSA, IP, WB
0.1 ml / 515,00 €
  OriGene Technologies GmbH

Heat shock factor 2 / HSF2 Antikörper

  Rat Monoclonal Anti-HSF2 Antibody  
Western blot analysis (K562 cells) transiently transfected with control,  HSF1 or HSF2 shRNA constructs using HSF1, HSF2 (3E2) antibodies. Courtesy of Lea Sistonen, Abo Akademi University, Finland. Rat IgG1 3E2 Purified var. C, E, EMSA, ICC/IF, IP, WB
0.1 mg / 330,00 €
  OriGene Technologies, Inc.

Heat shock factor 2 / HSF2 Antikörper

Western blot analysis (K562 cells) transiently transfected with control, HSF1 or HSF2 shRNA constructs using HSF1, HSF2 (3E2) antibodies. Courtesy of Lea Sistonen, Abo Akademi University, Finland. Rat IgG1 3E2 Purified var. C, E, EMSA, ICC/IF, IP, WB
25 µg / 190,00 €
  OriGene Technologies GmbH

MAF Antikörper

Recombinant Maf-GST protein Rabbit Serum Hu C, E, EMSA, WB
0.2 ml / 430,00 €
  OriGene Technologies GmbH

NF-kB p105 / p50 Antikörper

  Rabbit polyclonal anti-NFKB p50 antibody  
All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature.  The membrane was blocked in 5% dry milk for 2 h.  After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h.  Washes with buffer were performed 4 times for 5' each.  The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h.   Washes with TBS preceded color development. Rabbit Serum Hu E, EMSA, IP, WB
0.1 ml / 515,00 €
  OriGene Technologies, Inc.

NFYA Antikörper

  Rabbit polyclonal anti-NF-Y antibody  
WB of Rabbit Anti-NF-Y(A subunit) Antibody. Lane 1: CHO-7 cells in the absence (I) of cholesterol. Lane 2: CHO-7 cells in the presence (S) of cholesterol. Load: Equivalent aliquots of chromatin from each sample containing ~50 µg of total protein per lane. Primary antibody: anti-NF-YA antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Rabbit Purified Hu, Ms E, EMSA, IP, WB
0.1 mg / 515,00 €
  OriGene Technologies, Inc.

NFYA Antikörper

  Rabbit polyclonal anti-NF-Y antibody  
WB of Rabbit Anti-NF-Y (A Subunit) Antibody. Lane 1: CHO-7 cells were cultured in the absence (I) of cholesterol. Lane 2: CHO-7 cells were cultured in the presence (S) of cholesterol. Load: 35 µg per lane. Primary antibody: NF-YA antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Rabbit Serum Hu, Ms E, EMSA, IP, WB
0.1 ml / 515,00 €
  OriGene Technologies, Inc.

POU2F1 / OCT1 Antikörper

Western blot analysis of Oct-1 expression in Jurkat (Lane 1) and MCF-7 (Lane 2) using AM20290AF-N Oct1 antibody. Mouse IgG1 YL15 Azide Free Hu, Ms EMSA, IP, WB
0.1 mg / 375,00 €
  OriGene Technologies GmbH

REL Antikörper

  Rabbit polyclonal NFkB cRel antibody  
WB of HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The WB was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development. Rabbit Serum Hu E, EMSA, IP, WB
0.1 ml / 515,00 €
  OriGene Technologies, Inc.

RELA / NF-kB p65 Antikörper

  Rabbit polyclonal anti-NFKB p65 antibody  
Western Blot of Rabbit anti-NFkB p65 (Rel A) antibody. Lane 1: HeLa cell extract. Lane 2: HeLa cell extract. Load: 35 µg per lane. Primary antibody: NFkB p65 Rel A antibody at 1:5000 for 2 H at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:2000 for 60 min at RT. Block: 5% BLOTTO 2 H at RT. Predicted/Observed size: ~65 kDa, ~65 kDa for NFkb p65 Rel A. Other band(s): None. Rabbit Serum Hu, Ms, Rt E, EMSA, IP, WB
0.1 ml / 515,00 €
  OriGene Technologies, Inc.

RELA / NF-kB p65 Antikörper

Western blot analysis of NFkB IP samples. IP was performed on Jurkat cell lysates using 10 µg (lanes 2 & 7), 5 µg (lanes 3 & 8), 1 µg (lanes 4 & 9) Sheep anti NFkB p65 antibody (SP2092P), and 10 µg polyclonal IgG (lanes 5 & 10). Sheep Aff - Purified Hu E, EMSA, IP, WB
0.1 mg / 590,00 €
  OriGene Technologies GmbH

RFX5 Antikörper

  Rabbit polyclonal anti-RFX5 antibody  
Western Blot of Rabbit Anti-RFX5 antibody. Lane 1: Raji B cell nuclear extract lysates. Lane 2: none. Load: 35 µg per lane. Primary antibody: RFX5 antibody at 1:2,500 for overnight at 4°C. Secondary antibody: HRP Goat-a-Rabbit IgG secondary antibody at 1:5,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 65.3 kDa, 72 kDa for RFX5. Other band(s): none. Rabbit Ig Fraction Hu, Ms E, EMSA, IP, WB
0.1 mg / 515,00 €
  OriGene Technologies, Inc.

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