TA309071 14-3-3 protein beta/alpha (C-term) Antikörper

Rabbit Anti-14-3-3 Protein Antibody

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0.1 ml / 405,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Kurzübersicht

Rabbit anti Bovine, Canine, Chicken, Human, Mouse, Primate, Rat, Xenopus, Zebrafish 14-3-3 protein beta/alpha

Produktbeschreibung für 14-3-3 protein beta/alpha

Rabbit anti Bovine, Canine, Chicken, Human, Mouse, Primate, Rat, Xenopus, Zebrafish 14-3-3 protein beta/alpha.
Properties: (C-term)
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Produktdaten von 14-3-3 protein beta/alpha

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 0.1 ml
Synonyme KCIP-1, Protein 1054, Protein kinase C inhibitor protein 1, YWHAB
Präsentation Aff - Purified
Reaktivität Bov, Can, Chk, Hu, Ms, Prim, Rt, Xen, Ze
Anwendungen WB
Klonalität Polyclonal
Wirt Rabbit
Isotype IgG
Shipping to Europe, USA/Canada
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies, Inc.

Datenblatt-Auszug

Immunogen
Swiss Prot Num:
P35213
Immunogen:
Peptide corresponding to amino acid residues from the C-terminal region of 14-3-3 protein, conjugated to keyhole limpet hemocyanin (KLH).
GeneID:
56011
Eigenschaften C-term
Anwendung Western Blot: 1/1000.
Background 14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004; Dougherty and Morrison, 2004). The 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking.
Product Citations
Originator or purchased from resellers:
  1. Surgucheva I, Shestopalov VI, Surguchov A. Effect of gamma-synuclein silencing on apoptotic pathways in retinal ganglion cells. J Biol Chem. 2008 Dec 26;283(52):36377-85. doi: 10.1074/jbc.M806660200. Epub 2008 Oct 20. PubMed PMID: 18936092. (Free PMC Article available, 7 images available)
General Readings
  1. Berg D, Holzmann C, Riess O. 14-3-3 proteins in the nervous system. Nat Rev Neurosci. 2003 Sep;4(9):752-62. PubMed PMID: 12951567.
  2. Bridges D, Moorhead GB. 14-3-3 proteins: a number of functions for a numbered protein. Sci STKE. 2005 Aug 9;2005(296):re10. PubMed PMID: 16091624.
  3. Dougherty MK, Morrison DK. Unlocking the code of 14-3-3. J Cell Sci. 2004 Apr 15;117(Pt 10):1875-84. PubMed PMID: 15090593.
  4. Silhan J, Obsilova V, Vecer J, Herman P, Sulc M, Teisinger J, et al. 14-3-3 protein C-terminal stretch occupies ligand binding groove and is displaced by phosphopeptide binding. J Biol Chem. 2004 Nov 19;279(47):49113-9. Epub 2004 Sep 4. PubMed PMID: 15347690.
Storage Store the antibody undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography.
Buffer System:
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% Glycerol
State:
Liquid purified Ig fraction.
Aff - Purified
Species Reactivity
Species reactivity (expected):
Human, Bovine, Canine, Chicken, Mouse, non-Human Primates, Xenopus and Zebrafish.
Species reactivity (tested):
Rat.
Specificity
Specificity:
Specific for the ~29k 14-3-3 protein.
Immunolabeling of the 14-3-3 protein band is completely blocked by pre-adsorption of the antibody with the C-terminal peptide that was used to generate the antibody.

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