AP05566SU-N 14-3-3 protein eta Antikörper

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0.1 ml / 410,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Kurzübersicht

Rabbit anti Bovine, Chicken, Human, Mouse, Rabbit, Rat, Sheep 14-3-3 protein eta

Produktbeschreibung für 14-3-3 protein eta

Rabbit anti Bovine, Chicken, Human, Mouse, Rabbit, Rat, Sheep 14-3-3 protein eta.
Presentation: Serum
Product is tested for Western blot / Immunoblot, Paraffin Sections.

Produktdaten von 14-3-3 protein eta

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 0.1 ml
Synonyme Protein AS1, YWHA1, YWHAH
Präsentation Serum
Reaktivität Bov, Chk, Hu, Ms, Rb, Rt, Sh
Anwendungen P, WB
Klonalität Polyclonal
Wirt Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datenblatt-Auszug

Immunogen
Swiss Prot Num:
Q04917
Immunogen:
Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 eta.
AA Sequence:
Ac.GDREQLLQRARC
GeneID:
7533
Remarks:
Antiserum Preparation: Antisera to sheep 14-3-3 eta were raised by repeated immunisations of rabbit with highly purified antigen.
Anwendung Western Blot: Detects a band of approximately 30kDa in HEK293 cell lysates.
Immunohistochemistry on Paraffin Sections: 1/3200. Requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Background 14-3-3 eta is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.
14-3-3 eta is expressed principally in the brain although it is expressed at low levels in other tissues. The protein binds alphasynuclein, forming part of lewy bodies in parkinsons disease and has been linked to earlyonset schizophrenia. The eta isoform binds both gremlin 1, which is overexpressed in human cancers, and the DAL1/Protein 4.1B tumour suppressor. Furthermore the glucocortcoid receptor is positively regulated by 14-3-3eta.
AHP1046 may not react with recombinant proteins that are not Nacetylated.
General Readings
  1. Martin, H. et al. (1993) Antibodies against major brain isoforms of 1433 protein. An antibody specific for the Nacetylated aminoterminus of a protein. FEBS 331: 296 - 303.
  2. Aitken, A. et al. (2006) 1433 proteins: A historic overview. Seminars in Cancer Biology 16: 162 - 172.
  3. Aitken, A. et al. (2006) 14-3-3 proteins: A historic overview. Seminars in Cancer Biology 16: 162-172.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
State:
Liquid Serum containing 0.09% Sodium Azide as preservative
Serum
Species Reactivity
Species reactivity (expected):
Mammals.
Species reactivity (tested):
Sheep, Mouse, Human, Bovine, Chicken, Rabbit, Rat.
Specificity
Specificity:
This antibody recognises 14-3-3 eta in all mammals.
This antibody may not react with recombinant proteins that are not N-acetylated.
Gene ID 7533
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteine zu 14-3-3 protein eta (9 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

14-3-3 protein eta

14-3-3 protein eta Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / 748,00 €
  OriGene Technologies, Inc.

14-3-3 protein eta (1-246, His-tag)

14-3-3 eta, 1-246 aa, His-tagged: 15% SDS-PAGE (4 µg) Human Purified ≥ 95 by SDS-PAGE E. coli
0.5 mg / 820,00 €
  OriGene Technologies GmbH

14-3-3 protein eta (1-246, His-tag)

14-3-3 eta, 1-246 aa, His-tagged: 15% SDS-PAGE (4 µg) Human Purified ≥ 95 by SDS-PAGE E. coli
0.1 mg / 320,00 €
  OriGene Technologies GmbH

14-3-3 protein eta (1-246)

14-3-3 protein eta Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / 820,00 €
  OriGene Technologies GmbH

14-3-3 protein eta (1-246)

14-3-3 protein eta Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / 320,00 €
  OriGene Technologies GmbH

14-3-3 protein eta

14-3-3 protein eta Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

14-3-3 protein eta

14-3-3 protein eta Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

14-3-3 protein eta

14-3-3 protein eta Human
  Abnova Taiwan Corp.

14-3-3 protein eta

SDS-Page: 14-3-3 eta Protein [NBC1-18397] - 14-3-3 eta, 30.3 kDa (266 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE Protein
  Novus Biologicals Inc.

Positive controls for 14-3-3 protein eta (1 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

14-3-3 eta Lysate

Western Blot: 14-3-3 eta Lysate [NBL1-17950] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for YWHAH
  Novus Biologicals Inc.
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