AP22919PU-N 4E-BP2 (99-120) Antikörper

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50 µg / 425,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Kurzübersicht

Rabbit anti Bovine, Canine, Human, Mouse, Porcine, Rat 4E-BP2

Produktbeschreibung für 4E-BP2

Rabbit anti Bovine, Canine, Human, Mouse, Porcine, Rat 4E-BP2.
Properties: (99-120)
Presentation: Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Produktdaten von 4E-BP2

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 50 µg
Synonyme EIF4EBP2, Eukaryotic translation initiation factor 4E-binding protein 2, eIF4E-binding protein 2
Präsentation Purified
Reaktivität Bov, Can, Hu, Ms, Por, Rt
Anwendungen P, WB
Klonalität Polyclonal
Wirt Rabbit
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datenblatt-Auszug

Immunogen
Swiss Prot Num:
Q13542
Immunogen:
EIF4EBP2 antibody was raised against human PHAS-II synthetic peptide (residues 99-120) was synthesized and the peptide coupled to KLH.
GeneID:
1979
Remarks:
This sequence is 96% homologous to mouse PHAS-11 over these residues
Eigenschaften 99-120
Anwendung Immunohistochemistry on Paraffin Sections: 10 µg/ml.
Western Blot: 4 µg/ml.
Background PHAS-II, also known as eIF4E-binding protein II (eIF4E-BPII), is a member of a family of proteins which regulate initiation. PHAS-I and -II were found to have overlapping but different patterns of expression in tissues. PHAS-II shows the highest expression in liver and kidney where very little PHAS-I is found. The PHAS proteins regulate translation initiation by binding to the inhibitory protein eIF- 4E and blocking translation by preventing access of eIF-4G to the 5' cap of the mRNA. Both PHAS proteins are phosphorylated in response to insulin or growth factors such as EGF, PDGF and IGF-1. Phosphorylation in the appropriate site(s) promotes dissociation of PHAS/eIF-4E complexes which allows eIF-4E to bind eIF-4G(p220), thereby increasing the amount of eIF-4F complex and the rate of translation initiation. Regulation of the two protein differ because PHAS-II, unlike PHAS-I is readily phosphorylated by PKA in vitro. However increasing cAMP in cells promotes dephosphorylation of both PHAS-I and PHAS-II. Pharmacological and genetic evidence indicates that the mTOR/p70S6K pathway is involved in the control of PHAS-I and -II suggesting that these proteins may be mediators of the effects of this pathway on protein synthesis and cell proliferation.
Concentration 1 mg/ml
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Immunoaffinity Chromatography
Buffer System:
BBS, pH 8.4 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA)
State:
Liquid purified Ig fraction
Purified
Species Reactivity
Species reactivity (expected):
Bovine, Canine, Porcine, Mouse and Rat.
Species reactivity (tested):
Human.
Specificity
Specificity:
Detects 17 and 20 kD proteins, corresponding to the apparent molecular mass of PHAS-II and its phosphorylated state on SDS-PAGE immunoblots.
Gene ID 1979

Accessory Products

Proteins and/or Positive Controls

Proteine zu 4E-BP2 (11 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

4E-BP2

4E-BP2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / 748,00 €
  OriGene Technologies, Inc.

4E-BP2

4E-BP2 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / 299,00 €
  OriGene Technologies, Inc.

4E-BP2 (1-120, His-tag)

Recombinant human EIF4EBP2, 1-120 aa, His-tagged Human Purified > 85 % E. coli
0.5 mg / 820,00 €
  OriGene Technologies GmbH

4E-BP2 (1-120, His-tag)

Recombinant human EIF4EBP2, 1-120 aa, His-tagged Human Purified > 85 % E. coli
0.1 mg / 320,00 €
  OriGene Technologies GmbH

4E-BP2

4E-BP2 Human in vitro transl.
  Abnova Taiwan Corp.

4E-BP2

4E-BP2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

4E-BP2

4E-BP2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

4E-BP2

4E-BP2 Human Purified 95 % E. coli
  GenWay Biotech Inc.

4E-BP2

4E-BP2 Human Purified 95 % E. coli
  GenWay Biotech Inc.

4E-BP2

4E-BP2 Human Purified
  Novus Biologicals Inc.

4E-BP2

4E-BP2 Human Purified
  Novus Biologicals Inc.

Positive controls for 4E-BP2 (1 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

eIF4EBP2 Lysate

Western Blot: eIF4EBP2 Lysate [NBL1-10207] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for EIF4EBP2
  Novus Biologicals Inc.
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