AM10029FC-N acidic Cytokeratin Antikörper

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0.2 mg / 470,00 €
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Kurzübersicht

Mouse anti Bovine, Chicken, Human, Monkey, Mouse, Porcine, Rabbit, Rat acidic Cytokeratin AE1

AM10029FC-N

Produktbeschreibung für acidic Cytokeratin

Mouse anti Bovine, Chicken, Human, Monkey, Mouse, Porcine, Rabbit, Rat acidic Cytokeratin AE1.
Presentation: FITC
Product is tested for Flow Cytometry, Immunocytochemistry/Immunofluorescence.

Produktdaten von acidic Cytokeratin

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 0.2 mg
Synonyme Cytokeratin type I, Keratin type I, LMW Cytokeratins
Präsentation FITC
Reaktivität Bov, Chk, Hu, Mky, Ms, Por, Rb, Rt
Anwendungen F, ICC/IF
Klonalität Monoclonal
Klon AE1
Wirt Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datenblatt-Auszug

Immunogen
Immunogen:
Human epithelial keratin.
Remarks:
Molecular weight of antigen: (CK 10 (56.5), CK14 (50), CK15 (50), CK16 (48) and CK19 (40).
Isotype control AM03095PU-N, SM10F (for use in human samples), SM20F (for use in rat samples)
Anwendung Immunofluorescence: 10-20 µg/ml (1/10-1/20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC.
Flow Cytometry: 0.2-1.0 µg/0.1 ml (1/200-1/1,000) (Not tested in our lab).
Background Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20.
Concentration 0.2 mg/ml
General Readings
  1. Moll R, Franke WW, Schiller DL, Geiger B, Krepler R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell. 1982 Nov;31(1):11-24. PubMed PMID: 6186379.
  2. Sun TT, Tseng SC, Huang AJ, Cooper D, Schermer A, Lynch MH, et al. Monoclonal antibody studies of mammalian epithelial keratins: a review. Ann N Y Acad Sci. 1985;455:307-29. PubMed PMID: 2417518.
  3. Spagnolo DV, Michie SA, Crabtree GS, Warnke RA, Rouse RV. Monoclonal anti-keratin (AE1) reactivity in routinely processed tissue from 166 human neoplasms. Am J Clin Pathol. 1985 Dec;84(6):697-704. PubMed PMID: 2416215.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
FITC
Purification:
Protein A Chromatography
Buffer System:
PBS, pH 7.4
Preservatives:
0.05% Sodium Azide
Stabilizers:
1% BSA
State:
Liquid purified Ig fraction
Purified
Specificity
Specificity:
This antibody recognizes low molecular weight cytokeratins (CK 10 (56.5), CK14 (50), CK15 (50), CK16 (48) and CK19(40) of the acidic family.
Cellular Localization: Cytoplasmic.
Species:
Human, Monkey, Bovine, Porcine, Rat, Mouse, Rabbit and Chicken.

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