AM10029PU-N acidic Cytokeratin Antikörper

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1 ml / 370,00 €
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Mouse anti Bovine, Chicken, Human, Monkey, Mouse, Porcine, Rabbit, Rat acidic Cytokeratin AE1

AM10029PU-N

Produktbeschreibung für acidic Cytokeratin

Mouse anti Bovine, Chicken, Human, Monkey, Mouse, Porcine, Rabbit, Rat acidic Cytokeratin AE1.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Paraffin Sections.

Produktdaten von acidic Cytokeratin

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 1 ml
Synonyme Cytokeratin type I, Keratin type I, LMW Cytokeratins
Präsentation Purified
Reaktivität Bov, Chk, Hu, Mky, Ms, Por, Rb, Rt
Anwendungen C, ICC/IF, P
Klonalität Monoclonal
Klon AE1
Wirt Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datenblatt-Auszug

Immunogen
Immunogen:
Human epithelial keratin.
Remarks:
Molecular weight of antigen: (CK 10 (56.5), CK14 (50), CK15 (50), CK16 (48) and CK19 (40).
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Anwendung Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears).
For IHC dilute concentared antibody at 1/50-1/100, use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature.
FFPE tissue section requires antigen retrieval (boiling tissue in 10 mM citrate, pH 6.0 for 10-15 minutes followed by cooling for 10-15 minutes).
Recommended Positive Control: Human skin, squamous cell Ca.
Background Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20.
Concentration 0.2 mg/ml
General Readings
  1. Moll, R et al Cell, 31: 11-24, (1982).
  2. Sun, TT et. al. Am. N Y Acad. Sci. 455: 307-329 (1985).
  3. Spagnolo, DV et. al.Am.J Pathol. 84: 697-704, 1985.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Purification:
Protein A Chromatography
Buffer System:
PBS, pH 7.4
Preservatives:
0.05% Sodium Azide
Stabilizers:
1% BSA
State:
Liquid purified Ig fraction
Purified
Specificity
Specificity:
This antibody recognizes low molecular weight cytokeratins (CK 10 (56.5), CK14 (50), CK15 (50), CK16 (48) and CK19(40) of the acidic family.
Cellular Localization: Cytoplasmic.
Species:
Human, Monkey, Bovine, Porcine, Rat, Mouse, Rabbit and Chicken.
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