AM33271PU-T acidic Cytokeratin Antikörper

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20 µg / 240,00 €
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Mouse anti Bovine, Canine, Chicken, Human, Monkey, Mouse, Rabbit, Rat acidic Cytokeratin SPM115

AM33271PU-T

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  • AM33271PU-T

Produktbeschreibung für acidic Cytokeratin

Mouse anti Bovine, Canine, Chicken, Human, Monkey, Mouse, Rabbit, Rat acidic Cytokeratin SPM115.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Frozen Sections, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Paraffin Sections.

Produktdaten von acidic Cytokeratin

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 20 µg
Synonyme Cytokeratin type I, Keratin type I, LMW Cytokeratins
Präsentation Purified
Reaktivität Bov, Can, Chk, Hu, Mky, Ms, Rb, Rt
Anwendungen C, F, ICC/IF, P, WB
Klonalität Monoclonal
Klon SPM115
Wirt Mouse
Isotype IgG1
Molecular weight 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19)
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datenblatt-Auszug

Immunogen
Immunogen:
Human epidermal keratin.
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Anwendung ELISA: Use BSA free Antibody for coating.
Western Blot:
0.5-1 µg/ml. 
Flow Cytometry:
0.5-1 µg/106 cells.
Immunofluorescence: 1-2 µg/ml.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. 
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 
Positive Control: Skin, Squamous Cell Carcinoma (SCC).
Background Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20.
Concentration 0.2 mg/ml
General Readings
  1. Woodcock-Mitchell J, Eichner R, Nelson WG, Sun TT. Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J Cell Biol. 1982 Nov;95(2 Pt 1):580-8. PubMed PMID: 6183275. (Free PMC Article available)
  2. Tseng SCG et. al. Cell 1982; 30361.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
10mM PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction from Bioreactor Concentrate
Purified
Species Reactivity
Species reactivity (tested):
Human, Monkey, Cow, Dog, Rabbit, Mouse, Rat, Chicken, Turtle.
Specificity
Specificity:
This Monoclonal Antibody (Clone SPM115) recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI < 5.7) and basic (pI > 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50’, 48, 46, 45, and 40kDa.
This SPM116 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. 
Cellular Localization: Cytoplasmic.

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