AM50166PU-S ACTA2 / aortic smooth muscle Actin Antikörper

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0.1 mg / 430,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Kurzübersicht

Mouse anti Bovine, Canine, Chicken, Feline, Guinea Pig, Goat, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Baboon ACTA2 / aortic smooth muscle Actin SPM332

Produktbeschreibung für ACTA2 / aortic smooth muscle Actin

Mouse anti Bovine, Canine, Chicken, Feline, Guinea Pig, Goat, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Baboon ACTA2 / aortic smooth muscle Actin SPM332.
Presentation: Purified
Product is tested for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Paraffin Sections, Western blot / Immunoblot.

Produktdaten von ACTA2 / aortic smooth muscle Actin

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 0.1 mg
Synonyme ACTSA, ACTVS, Alpha-actin-2, Cell growth-inhibiting gene 46 protein
Präsentation Purified
Reaktivität Bab, Bov, Can, Chk, Fe, GP, Gt, Hu, Mky, Ms, Por, Rb, Rt, Sh
Anwendungen F, ICC/IF, IP, P, WB
Klonalität Monoclonal
Klon SPM332
Wirt Mouse
Isotype IgG2a
Molecular weight 42kDa
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datenblatt-Auszug

Immunogen
Swiss Prot Num:
P62736
Immunogen:
N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH.
GeneID:
59
Isotype control AM03096PU-N
Anwendung Flow Cytometry: 0.5-1 μg/million cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blotting: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistology (Formalin-Fixed): 0.5-1.0 μg/ml for 30 minutes at RT.
(Staining of Formalin-Fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min.)
Positive Control: Blood vessels in all tissues, smooth muscle or leiomyosarcoma.
Concentration 0.2 mg/ml
General Readings
  1. Skalli O, Ropraz P, Trzeciak A, Benzonana G, Gillessen D, Gabbiani G. A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation. J Cell Biol. 1986 Dec;103(6 Pt 2):2787-96. PubMed PMID: 3539945. (Free PMC Article available)
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
10mM PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction from Bioreactor Concentrate
Purified
Specificity
Specificity:
Actin is a major component of the cytoskeleton and is present in most cell types.
This Monoclonal Antibody is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This  Monoclonal Antibody does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
Cellular Localization: Cytoplasmic.
Species:
Human, Baboon, Monkey, Cow, Pig, Sheep, Goat, Cat, Dog, Rabbit, Mouse, Rat, Guinea pig and Chicken.

Accessory Products

Proteins and/or Positive Controls

Proteine zu ACTA2 / aortic smooth muscle Actin (6 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

ACTA2 / aortic smooth muscle Actin (transcript variant 1)

ACTA2 / aortic smooth muscle Actin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / 748,00 €
  OriGene Technologies, Inc.

ACTA2 / aortic smooth muscle Actin (3-377, His-tag)

ACTA2 / aortic smooth muscle Actin Human Purified > 85 % by SDS - PAGE E. coli
0.25 mg / 1.070,00 €
  OriGene Technologies GmbH

ACTA2 / aortic smooth muscle Actin (3-377, His-tag)

ACTA2 / aortic smooth muscle Actin Human Purified > 85 % by SDS - PAGE E. coli
50 µg / 400,00 €
  OriGene Technologies GmbH

ACTA2 / aortic smooth muscle Actin

ACTA2 / aortic smooth muscle Actin Human
  Abnova Taiwan Corp.

ACTA2 / aortic smooth muscle Actin

ACTA2 / aortic smooth muscle Actin Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

ACTA2 / aortic smooth muscle Actin

ACTA2 / aortic smooth muscle Actin Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for ACTA2 / aortic smooth muscle Actin (1 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

Actin Alpha 2 Smooth Muscle Lysate

Western Blot: Actin Alpha 2 Smooth Muscle Lysate [NBL1-07272] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for ACTA2 Protein
  Novus Biologicals Inc.
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