TA306253 AES / ESP1 (C-term) Antikörper

Rabbit Polyclonal AES Antibody

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0.1 mg / 415,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Rabbit anti Human, Mouse, Rat AES / ESP1

TA306253

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Produktbeschreibung für AES / ESP1

Rabbit anti Human, Mouse, Rat AES / ESP1.
Properties: (C-term)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Produktdaten von AES / ESP1

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 0.1 mg
Synonyme AES1, Amino-terminal enhancer of split, GRG protein, Gp130-associated protein GAM
Präsentation Aff - Purified
Reaktivität Hu, Ms, Rt
Anwendungen E, ICC/IF, WB
Klonalität Polyclonal
Wirt Rabbit
Isotype IgG
Shipping to Europe, USA/Canada
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies, Inc.

Datenblatt-Auszug

Immunogen
Swiss Prot Num:
Q08117
Immunogen:
16 amino acid synthetic peptide from near the carboxy terminus of Human AES.
Human AES / Amino-terminal enhancer of split / GRG / ESP1 / TLE5 (C-Terminus) Peptide (Cat.-No AP30024CP-N)
GeneID:
166
Eigenschaften C-term
Add. information Blocking peptide available: AP30024CP-N
Anwendung ELISA.
Western Blot: AES antibody can be used for the detection of AES at 1-4 μg/ml. 
Positve Control: 293 cell lysate. 
Immunocytochemistry: Start at 10 µg/ml. 
Positve Control: HeLa cells. 
Immunofluorescence:
Start at 20 µg/ml. 
Positve Control: HeLa cells.
Background Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment (1). Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination (2,3). AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis (4). It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. (4). Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin (4).
Concentration 1.0 mg/ml
General Readings
  1. Martin SS, Vuori K. Regulation of Bcl-2 proteins during anoikis and amorphosis. Biochim Biophys Acta. 2004 Jul 5;1692(2-3):145-57. PubMed PMID: 15246684.
  2. Miyasaka H, Choudhury BK, Hou EW, Li SS. Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein. Eur J Biochem. 1993 Aug 15;216(1):343-52. PubMed PMID: 8365415.
  3. Chen G, Courey AJ. Groucho/TLE family proteins and transcriptional repression. Gene. 2000 May 16;249(1-2):1-16. PubMed PMID: 10831834.
  4. Jan Y, Matter M, Pai JT, Chen YL, Pilch J, Komatsu M, et al. A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and Groucho/TLE corepressors. Cell. 2004 Mar 5;116(5):751-62. PubMed PMID: 15006356.
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Purification:
Affinity Chromatography purified via peptide column
Buffer System:
PBS
Preservatives:
0.02% Sodium Azide
State:
Liquid purified Ig fraction
Aff - Purified
Specificity
Specificity:
AES antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human AES.
Species:
Human, Mouse, Rat.

Accessory Products

Proteins and/or Positive Controls

Proteine zu AES / ESP1 (10 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

AES / ESP1 (transcript variant 2)

AES / ESP1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / 748,00 €
  OriGene Technologies, Inc.

AES / ESP1 (transcript variant 3)

AES / ESP1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / 748,00 €
  OriGene Technologies, Inc.

AES / ESP1 (1-197, His-tag)

AES / ESP1 Human Purified > 95 % E. coli
0.25 mg / 820,00 €
  OriGene Technologies GmbH

AES / ESP1 (1-197, His-tag)

AES / ESP1 Human Purified > 95 % E. coli
50 µg / 320,00 €
  OriGene Technologies GmbH

AES / ESP1

AES / ESP1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

AES / ESP1

AES / ESP1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

AES / ESP1

AES / ESP1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

AES / ESP1

AES / ESP1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

AES / ESP1 (Groucho homolog)

AES / ESP1 Human Purified
  Novus Biologicals Inc.

AES / ESP1 (Groucho homolog)

AES / ESP1 Human Purified
  Novus Biologicals Inc.

Positive controls for AES / ESP1 (1 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

AES / ESP1 Lysate(Denatured)

AES / ESP1 Lysate(Denatured)
  Abnova Taiwan Corp.
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