R1054HRPS Aldolase A / ALDOA Antikörper

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0.1 mg / 515,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Kurzübersicht

Goat anti Human, Rabbit Aldolase A / ALDOA

Produktbeschreibung für Aldolase A / ALDOA

Goat anti Human, Rabbit Aldolase A / ALDOA.
Presentation: HRP
Product is tested for Immunoprecipitation, Enzyme Immunoassay, Western blot / Immunoblot.

Produktdaten von Aldolase A / ALDOA

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 0.1 mg
Synonyme ALDA, Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, NY-LU-1
Präsentation HRP
Reaktivität Hu, Rb
Anwendungen E, IP, WB
Klonalität Polyclonal
Wirt Goat
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datenblatt-Auszug

Immunogen
Swiss Prot Num:
P00883
Immunogen:
Aldolase from rabbit muscle
GeneID:
100009055
Anwendung Western blot:1/500-1/5,000.
Immunoprecipitation: 1/100.
Use approximately 5 µl of antibody to immunoprecipitate 50 µl of protein lysate.
ELISA: 1/5000-1/20,000.
This antibody has been assayed against 1.0 µg of aldolase [rabbit muscle] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/2,000 of the reconstitution concentration is suggested.
Caution Do NOT add Sodium azide!
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Horseradish peroxidase
HRP
Purification:
Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Buffer System:
0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
Preservatives:
0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
Stabilizers:
10 mg/ml BSA (immunoglobulin and protease free)
State:
Lyophilized purified Ig fraction.
Purified
Reconstitution:
Restore with 0.1 ml of deionized water (or equivalent).
Specificity
Specificity:
This antibody detects rabbit aldolase. Cross reactivity against aldolase from human tissues is known. Crossactivity against aldolase from other tissues and species may also occur.
Immunoelectrophoresis gives a single precipitin arc against anti-goat serum, purified and partially purified aldolase [Rabbit muscle].
It has been reported that this antibody can detect human Aldolase on immunoblot showing a 41 kDa band in lysates from MCF7, NMB231 and HBL100 cell lines.
Species:
Rabbit, Human.

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