BM4013 B-Cells (pan) Antikörper

See related secondary antibodies

Search for all "B-Cells"

50 µg / 480,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Kurzübersicht

Mouse anti Rat B-Cells Ki-B1R

Produktbeschreibung für B-Cells

Mouse anti Rat B-Cells Ki-B1R.
Properties: (pan)
Presentation: Purified
Product is tested for Frozen Sections, Flow Cytometry, Paraffin Sections.

Produktdaten von B-Cells

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 50 µg
Synonyme B cells, B-cell marker
Präsentation Purified
Reaktivität Rt
Anwendungen C, F, P
Klonalität Monoclonal
Klon Ki-B1R
Wirt Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datenblatt-Auszug

Immunogen
Immunogen:
Rat lymphocytes
Remarks:
The epitope has not been further characterized.
Eigenschaften pan
Isotype control AM03095PU-N, SM20P (for use in rat samples)
Anwendung Immunohistochemistry on Frozen sections: 1 µg/ml (1/100).
Immunohistochemistry on Paraffin sections: 10 µg/ml (1/10). No antigen retrieval required, microwave treatment may enhance the reactivity.
Suggested Positive Control: Rat spleen.
Has been described to work in FACS.
Background B cells are lymphocytes that play a large role in the humoral immune response as opposed to the cell-mediated immune response that is governed by T cells. The abbreviation "B" comes from bursa of Fabricius that is an organ in birds in which avian B cells mature. The principal function of B cells is to make antibodies against soluble antigens. B cells are an essential component of the adaptive immune system.
Concentration 0.1 mg/ml (after reconstitution)
Product Citations
Purchased from Acris:
  1. Schindewolffs L, Breves G, Buettner M, Hadamitzky C, Pabst R. VEGF-C improves regeneration and lymphatic reconnection of transplanted autologous lymph node fragments: An animal model for secondary lymphedema treatment. Immun Inflamm Dis. 2014 Nov;2(3):152-61. doi: 10.1002/iid3.32. Epub 2014 Nov 17. PubMed PMID: 25505549. (Free PMC Article available)
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography
Buffer System:
PBS, pH 7.2 with 10 mg/ml BSA as a stabilizer and 0.01% thimerosal as a preservative
State:
Lyohilized purified IgG fraction
Purified
Reconstitution:
Restore with 0.5 ml distilled water.
Specificity
Specificity:
Monoclonal Rat B-lymphocyte antibody BM4013 (clone Ki-B1R) is a pan B-cell marker useful for detecting B-lymphocytes and plasma cells in cell suspensions and tissue sections.
Ki-B1R recognises a surface antigen of 47 kD molecular weight, which appears as a single band under reducing conditions in SDS-PAGE.
The antibody is specific for Rat mature B-lymphocytes and plasma cells. Other species not tested.
Antigen Distribution:
Isolated Cells: In a test comparing the immunophenotype of rat blood lymphocytes, Ki-B1R recognised 19% +/- 3% of peripheral blood lymphocytes (n=5).
In the same test, 18% +/- 3% of the lymphocytes stained positive for sIg, whereas 79% +/- 3% stained positive with Ki-T1R, the rat pan T-cell marker.
Tissue Sections: Ki-B1R shows a staining pattern similar to that of anti-rat immunoglobulin markers. Follicular mantle B-lymphocytes are strongly positive whereas staining of germinal centre B-lymphocytes depends on the immunological status of the lyphoid organ. In the gastrointestinal tract, the typical mucosa-associated lymphoid tissue (MALT) selectively displays the B-follicles, mostly those containing prominent germinal centres. The reactivity pattern of Ki-B1R does not change when applied in vivo.
Species:
Rat (mature B-lymphocytes, plasma cells).

Accessory Products

  • LinkedIn