BM4013 B-Cells (pan) Antikörper

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50 µg / 480,00 €
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Mouse anti Rat B-Cells Ki-B1R

Produktbeschreibung für B-Cells

Mouse anti Rat B-Cells Ki-B1R.
Properties: (pan)
Presentation: Purified
Product is tested for Frozen Sections, Flow Cytometry, Paraffin Sections.

Produktdaten von B-Cells

Produkt-Kategorie Primärantikörper
Menge 50 µg
Synonyme B cells, B-cell marker
Präsentation Purified
Reaktivität Rt
Anwendungen C, F, P
Klonalität Monoclonal
Klon Ki-B1R
Wirt Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)


Rat lymphocytes
The epitope has not been further characterized.
Eigenschaften pan
Isotype control AM03095PU-N, SM20P (for use in rat samples)
Anwendung Immunohistochemistry on Frozen sections: 1 µg/ml (1/100).
Immunohistochemistry on Paraffin sections: 10 µg/ml (1/10). No antigen retrieval required, microwave treatment may enhance the reactivity.
Suggested Positive Control: Rat spleen.
Has been described to work in FACS.
Background B cells are lymphocytes that play a large role in the humoral immune response as opposed to the cell-mediated immune response that is governed by T cells. The abbreviation "B" comes from bursa of Fabricius that is an organ in birds in which avian B cells mature. The principal function of B cells is to make antibodies against soluble antigens. B cells are an essential component of the adaptive immune system.
Concentration 0.1 mg/ml (after reconstitution)
Product Citations
Purchased from Acris:
  1. Schindewolffs L, Breves G, Buettner M, Hadamitzky C, Pabst R. VEGF-C improves regeneration and lymphatic reconnection of transplanted autologous lymph node fragments: An animal model for secondary lymphedema treatment. Immun Inflamm Dis. 2014 Nov;2(3):152-61. doi: 10.1002/iid3.32. Epub 2014 Nov 17. PubMed PMID: 25505549. (Free PMC Article available)
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity chromatography
Buffer System:
PBS, pH 7.2 with 10 mg/ml BSA as a stabilizer and 0.01% thimerosal as a preservative
Lyohilized purified IgG fraction
Restore with 0.5 ml distilled water.
Monoclonal Rat B-lymphocyte antibody BM4013 (clone Ki-B1R) is a pan B-cell marker useful for detecting B-lymphocytes and plasma cells in cell suspensions and tissue sections.
Ki-B1R recognises a surface antigen of 47 kD molecular weight, which appears as a single band under reducing conditions in SDS-PAGE.
The antibody is specific for Rat mature B-lymphocytes and plasma cells. Other species not tested.
Antigen Distribution:
Isolated Cells: In a test comparing the immunophenotype of rat blood lymphocytes, Ki-B1R recognised 19% +/- 3% of peripheral blood lymphocytes (n=5).
In the same test, 18% +/- 3% of the lymphocytes stained positive for sIg, whereas 79% +/- 3% stained positive with Ki-T1R, the rat pan T-cell marker.
Tissue Sections: Ki-B1R shows a staining pattern similar to that of anti-rat immunoglobulin markers. Follicular mantle B-lymphocytes are strongly positive whereas staining of germinal centre B-lymphocytes depends on the immunological status of the lyphoid organ. In the gastrointestinal tract, the typical mucosa-associated lymphoid tissue (MALT) selectively displays the B-follicles, mostly those containing prominent germinal centres. The reactivity pattern of Ki-B1R does not change when applied in vivo.
Rat (mature B-lymphocytes, plasma cells).

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