SM1303F B-Cells (FMC7 Antigen) Antikörper

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100 Tests / 710,00 €
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Mouse anti Human B-Cells FMC7

Produktbeschreibung für B-Cells

Mouse anti Human B-Cells FMC7.
Properties: (FMC7 Antigen)
Presentation: FITC
Product is tested for Flow Cytometry.

Produktdaten von B-Cells

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 100 Tests
Synonyme B cells, B-cell marker
Präsentation FITC
Reaktivität Hu
Anwendungen F
Klonalität Monoclonal
Klon FMC7
Wirt Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datenblatt-Auszug

Immunogen
Immunogen:
HRIK cells - Human B-Lymphoblastoid line.
Eigenschaften FMC7 Antigen
Isotype control SM13F
Anwendung Flow Cytometry: Use 10 µl of neat antibody to label 10e6 cells or 100 µl whole blood.
Background The FMC7 antigen is expressed by peripheral B lymphocytes, and has been used widely in the study of B cell malignancy. FMC7 has been used extensively to differentiate various types of B cell malignancy. The nature of the FMC7 antigen has remained poorly defined following its first description in 1981.
General Readings 1. Brooks D.A., et al. (1981) Human lymphocyte markers defined by antibodies derived from somatic cell hybrids. A monoclonal antibody reacting specifically with a subpopulation of human B lymphocytes. J. Immunol.
126: 1373.
2. Catovsky D., et al. (1981) Heterogeneity of B cell leukaemias demonstrated by the monoclonal antibody
FMC7. Br. J. Haematol. 49: 137.
3. Zola H., et al. (1984) The human B cell lineage studied with monoclonal antibodies. In Leucocyte Typing Ed.A. Bernard, Springer Verlag. p363-371.
4. Zola H., et al. (1984) The antigen of mature human B cells detected by the monoclonal antibody FMC7; studies on the nature of the antigen and its expression. J. Immunol. 133: 321-326.
5. Bloem A.C., et al. (1988) Functional properties of human B cell populations defined by monoclonal antibodies HBA and FMC7. J. Immunol. 140: 768-773.
6. Zola H., et al. (1987) Markers of differentiated B cell leukemia: CD22 antibodies and FMC7 react with different molecules. Dis. Markers 5: 227.
7. Melo I.V., et al. (1988) The different diagnosis between chronic lymphocytic leukemia and other B-cell lymphoproliferative disorders: morphological and immunological studies. In: Chronic Lymphocytic Leukemia (eds. A. Polliack and D. Catovsky) p85. Harwood acad. Publ. London.
8. Ferro, L.M. and Zola H. (1990) Modulation of expression of the antigen identified by FMC7 upon human Blymphocyte activation: evidence for difference between activation in vivo and in vitro. Immunology. 69: 373-378.
9. Collins R.J., et al. (1992) Malignant lymphoma: reactive with the monoclonal antibody FMC7. Pathology 15: 350.
10. Serke, S. et al. (2001) Monoclonal antibody FMC7 detects a conformational epitope on the CD20 molecule:Evidence from phenotyping after Rituxan therapy and transfectant cell analyses. Cytometry (Comm.
Clin. Cytometry) 46:98-104
Storage Store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Format
Label:
Fluorescein Isothiocyanate Isomer 1
FITC
Purification:
Gel Filtration
Buffer System:
TRIS buffered glycine containing 0.09% Sodium Azide and 0.2% BSA
State:
Liquid purified IgM
Specificity
Specificity:
This antibody recognizes a glycoprotein antigen of 105kD expressed by B lymphocytes. B-CLL is generally considered to be negative for FMC7 expression, but strong staining is seen in many other types of B cell lymphoma, including prolymphocytic leukaemia and hairy cell leukaemia. The expression pattern closely corresponds to that seen with CD22, but studies have shown that FMC7 does not recognise the CD22 molecule itself. A recent publication by Serke et al has shown that FMC7 recognises a conformational epitope on the CD20 molecule, most likely a multimeric complex of CD20.
Species:
Human.

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