SM3010F CD15 Antikörper

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100 Tests / 260,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Kurzübersicht

Mouse anti Human CD15 MEM-158

Produktbeschreibung für CD15

Mouse anti Human CD15 MEM-158.
Presentation: FITC
Product is tested for Flow Cytometry.

Produktdaten von CD15

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 100 Tests
Synonyme Lacto-N-Fucopentaose III, Lewis X, SSEA1, Stage-Specific Embryonic Antigen, X-Hapten
Präsentation FITC
Reaktivität Hu
Anwendungen F
Klonalität Monoclonal
Klon MEM-158
Wirt Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datenblatt-Auszug

Immunogen
Immunogen:
Human granulocytes
Isotype control SM13F
Anwendung Suitable for Flow cytometry ( Use 20 µl to label 10e6 cells or 100 µl whole blood).
Background CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.
General Readings
  1. Benharroch D, Dima E, Levy A, Ohana-Malka O, Ariad S, Prinsloo I, Mejirovsky E, Sacks M, Gopas J: Differential expression of sialyl and non-sialyl-CD15 antigens on Hodgkin-Reed-Sternberg cells: significance in Hodgkin's disease. Leuk Lymphoma. 2000 Sep;39(1-2):185-94.
  2. Hakomori S: Le(X) and related structures as adhesion molecules. Histochem J. 1992 Nov;24(11):771-6.
  3. Li C, Wong P, Pan T, Xiao F, Yin S, Chang B, Kang SC, Ironside J, Sy MS: Normal cellular prion protein is a ligand of selectins: binding requires Le(X) but is inhibited by sLe(X). Biochem J. 2007 Sep 1;406(2):333-41.
  4. Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
Storage Store the antibody undiluted at 4-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Should it contain a precipitate we recommend microcentrifugation before use. Shelf life: one year from despatch.
Format
Label:
Conjugated with Fluorescein isothiocyanate under optimum conditions. The reagent is free of unconjugated
FITC
Buffer System:
Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent
State:
Liquid purified Ig fraction
Specificity
Specificity:
The antibody reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells.
Species:
Human.

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