AM12006AF-N CD28 Antikörper

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0.1 mg / 210,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Mouse anti Human, Primate CD28 CD28.2

AM12006AF-N

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  • AM12006AF-N

Produktbeschreibung für CD28

Mouse anti Human, Primate CD28 CD28.2.
Presentation: Azide Free
Product is tested for Frozen Sections, Functional assay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Flow Cytometry.

Produktdaten von CD28

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 0.1 mg
Synonyme T-cell-specific surface glycoprotein CD28, TP44
Präsentation Azide Free
Reaktivität Hu, Prim
Anwendungen C, F, FN, ICC/IF, IP, WB
Klonalität Monoclonal
Klon CD28.2
Wirt Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datenblatt-Auszug

Immunogen
Swiss Prot Num:
P10747
Immunogen:
DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA
GeneID:
940
Anwendung Western blot.
Immunoprecipitation.
Flow Cytometry.
Immunocytochemistry.
Functional Application:
 T cell costimulation.
Immunohistochemistry on Frozen Sections.
Background CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Concentration 1.0 mg/ml
General Readings
  1. Nunès J, Klasen S, Ragueneau M, Pavon C, Couez D, Mawas C, et al. CD28 mAbs with distinct binding properties differ in their ability to induce T cell activation: analysis of early and late activation events. Int Immunol. 1993 Mar;5(3):311-5. PubMed PMID: 8385476.
  2. Nunes J, Klasen S, Franco MD, Lipcey C, Mawas C, Bagnasco M, et al. Signalling through CD28 T-cell activation pathway involves an inositol phospholipid-specific phospholipase C activity. Biochem J. 1993 Aug 1;293 ( Pt 3):835-42. PubMed PMID: 8394695. (Free PMC Article available)
  3. Schlossman, S., et al., Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  4. Galea-Lauri J, Darling D, Gan SU, Krivochtchapov L, Kuiper M, Gäken J, et al. Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells. J Immunol. 1999 Jul 1;163(1):62-70. PubMed PMID: 10384100.
  5. Tazi A, Moreau J, Bergeron A, Dominique S, Hance AJ, Soler P. Evidence that Langerhans cells in adult pulmonary Langerhans cell histiocytosis are mature dendritic cells: importance of the cytokine microenvironment. J Immunol. 1999 Sep 15;163(6):3511-5. PubMed PMID: 10477625.
  6. Marti F, Krause A, Post NH, Lyddane C, Dupont B, Sadelain M, et al. Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6. J Immunol. 2001 Jan 1;166(1):197-206. PubMed PMID: 11123293.
  7. Scharschmidt E, Wegener E, Heissmeyer V, Rao A, Krappmann D. Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling. Mol Cell Biol. 2004 May;24(9):3860-73. PubMed PMID: 15082780. (Free PMC Article available, 9 images available)
  8. Jeong SH, Qiao M, Nascimbeni M, Hu Z, Rehermann B, Murthy K, et al. Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates. J Virol. 2004 Jul;78(13):6995-7003. PubMed PMID: 15194776. (Free PMC Article available, 3 images available)
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein A
Buffer System:
Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized
Preservatives:
None
Endotoxin level:
Less than 0.01 EU/μg of the protein, as determined by the LAL test
State:
Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Azide Free
Species Reactivity
Species reactivity (tested):
Human, Non-Human Primates
Specificity
Specificity:
The antibody reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
Gene ID 940

Accessory Products

Proteins and/or Positive Controls

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Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
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CD28 (C-term Fc tag)

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Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
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