SM1092FT CD32 / FcRII-a Antikörper

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25 µg / 180,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Kurzübersicht

Mouse anti Canine, Human, Monkey CD32 / FcRII-a AT10

Produktbeschreibung für CD32 / FcRII-a

Mouse anti Canine, Human, Monkey CD32 / FcRII-a AT10.
Presentation: FITC
Product is tested for Flow Cytometry.

Produktdaten von CD32 / FcRII-a

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 25 µg
Synonyme CDw32, FCG2, FCGR2A, FCGR2A1, Fc-gamma RII-a, Fc-gamma-RIIa, IGFR2, IgG Fc receptor II, Low affinity immunoglobulin gamma Fc region receptor II
Präsentation FITC
Reaktivität Can, Hu, Mky
Anwendungen F
Klonalität Monoclonal
Klon AT10
Wirt Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datenblatt-Auszug

Immunogen
Swiss Prot Num:
P12318
Immunogen:
K562 cell line.
GeneID:
2212
Isotype control SM10F (for use in human samples)
Anwendung Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells or 100 µl whole blood.
Background Human CD32 antigen is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets.
Concentration 0.1 mg/ml
General Readings
  1. Greenman J, Tutt AL, George AJ, Pulford KA, Stevenson GT, Glennie MJ. Characterization of a new monoclonal anti-Fc gamma RII antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors. Mol Immunol. 1991 Nov;28(11):1243-54. PubMed PMID: 1835758.
  2. Van Den Herik-Oudijk IE, Westerdaal NA, Henriquez NV, Capel PJ, Van De Winkel JG. Functional analysis of human Fc gamma RII (CD32) isoforms expressed in B lymphocytes. J Immunol. 1994 Jan 15;152(2):574-85. PubMed PMID: 8283039.
  3. Lilliehöök I, Johannisson A, Håkansson L. Expression of adhesion and Fcgamma-receptors on canine blood eosinophils and neutrophils studied by anti-human monoclonal antibodies. Vet Immunol Immunopathol. 1998 Feb 27;61(2-4):181-93. PubMed PMID: 9613433.
  4. Larsson M, Berge J, Johansson AG, Forsum U. Human dendritic cells handling of binding, uptake and degradation of free and IgG-immune complexed dinitrophenylated human serum albumin in vitro. Immunology. 1997 Jan;90(1):138-46. PubMed PMID: 9038724. (Free PMC Article available)
  5. Dutertre CA, Bonnin-Gélizé E, Pulford K, Bourel D, Fridman WH, Teillaud JL. A novel subset of NK cells expressing high levels of inhibitory FcgammaRIIB modulating antibody-dependent function. J Leukoc Biol. 2008 Dec;84(6):1511-20. doi: 10.1189/jlb.0608343. Epub 2008 Aug 21. PubMed PMID: 18719017.
  6. Devriendt B, Verdonck F, Summerfield A, Goddeeris BM, Cox E. Targeting of Escherichia coli F4 fimbriae to Fcgamma receptors enhances the maturation of porcine dendritic cells. Vet Immunol Immunopathol. 2010 Jun 15;135(3-4):188-98. doi: 10.1016/j.vetimm.2009.11.013. Epub 2009 Dec 3. PubMed PMID: 20022123.
  7. Sims GP, Ettinger R, Shirota Y, Yarboro CH, Illei GG, Lipsky PE. Identification and characterization of circulating human transitional B cells. Blood. 2005 Jun 1;105(11):4390-8. Epub 2005 Feb 8. PubMed PMID: 15701725. (Free PMC Article available, 7 images available)
  8. Benitez-Ribas D, Adema GJ, Winkels G, Klasen IS, Punt CJ, Figdor CG, et al. Plasmacytoid dendritic cells of melanoma patients present exogenous proteins to CD4+ T cells after Fc gamma RII-mediated uptake. J Exp Med. 2006 Jul 10;203(7):1629-35. Epub 2006 Jun 19. PubMed PMID: 16785312. (Free PMC Article available, 5 images available)
  9. Zhao, X.W. et al. (2011) CD47-signal regulatory protein-a (SIRPa) interactions form a barrier for antibody-mediated tumor cell destruction. Proc Natl Acad Sci U S A. Oct 31. [Epub ahead of print]
  10. Araújo MS, de Andrade RA, Sathler-Avelar R, Magalhães CP, Carvalho AT, Andrade MC, et al. Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis. Vet Immunol Immunopathol. 2011 May 15;141(1-2):64-75. doi: 10.1016/j.vetimm.2011.02.007. Epub 2011 Feb 23. PubMed PMID: 21439654.
  11. Santer DM, Hall BE, George TC, Tangsombatvisit S, Liu CL, Arkwright PD, et al. C1q deficiency leads to the defective suppression of IFN-alpha in response to nucleoprotein containing immune complexes. J Immunol. 2010 Oct 15;185(8):4738-49. doi: 10.4049/jimmunol.1001731. Epub 2010 Sep 15. PubMed PMID: 20844193. (Free PMC Article available, 6 images available)
  12. Shannon O, Mörgelin M, Rasmussen M. Platelet activation and biofilm formation by Aerococcus urinae, an endocarditis-causing pathogen. Infect Immun. 2010 Oct;78(10):4268-75. doi: 10.1128/IAI.00469-10. Epub 2010 Aug 9. PubMed PMID: 20696834. (Free PMC Article available, 6 images available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Fluorescein Isothiocyanate Isomer 1
FITC
Purification:
Affinity Chromatography on Protein A
Buffer System:
PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
State:
Liquid purified IgG fraction.
Specificity
Specificity:
The antibody recognizes the Human CD32 antigen.
Clone AT10 blocks the binding of IgG to Fc gamma R11 (See Ref. 4).
The use of SM1092LE is recommended for this purpose.
Species:
Human, Rhesus monkey, Pig and Dog .
Gene ID 2212

Accessory Products

Proteins and/or Positive Controls

Proteine zu CD32 / FcRII-a (10 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

CD32 / FcRII-a (transcript variant 2)

CD32 / FcRII-a Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / 748,00 €
  OriGene Technologies, Inc.

CD32 / FcRII-a (transcript variant 2)

CD32 / FcRII-a Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / 299,00 €
  OriGene Technologies, Inc.

CD32 / FcRII-a (34-216, His-tag)

CD32 / FcRII-a Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / 820,00 €
  OriGene Technologies GmbH

CD32 / FcRII-a (34-216, His-tag)

CD32 / FcRII-a Human Purified > 90 % by SDS - PAGE E. coli
20 µg / 320,00 €
  OriGene Technologies GmbH

CD32 / FcRII-a

CD32 / FcRII-a Human
  Abnova Taiwan Corp.

CD32 / FcRII-a

CD32 / FcRII-a Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD32 / FcRII-a

CD32 / FcRII-a Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD32 / FcRII-a

CD32 / FcRII-a Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD32 / FcRII-a

CD32 / FcRII-a Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD32 / FcRII-a

CD32 / FcRII-a Human
  Abnova Taiwan Corp.

Positive controls for CD32 / FcRII-a (2 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

FCGR2A Lysate

Western Blot: FCGR2A Lysate [NBL1-10655] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for FCGR2A
  Novus Biologicals Inc.

FCGR2A overexpression lysate

FCGR2A overexpression lysate
0.1 mg / 295,00 €
  OriGene Technologies, Inc.
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