AM26029RP-N CD4 Antikörper

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0.1 mg / 200,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.


Rat anti Mouse CD4 GK1.5


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  • AM26029RP-N
  • AM26029RP-N

Produktbeschreibung für CD4

Rat anti Mouse CD4 GK1.5.
Presentation: PE
Product is tested for Flow Cytometry.

Produktdaten von CD4

Produkt-Kategorie Primärantikörper
Menge 0.1 mg
Synonyme T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Präsentation PE
Reaktivität Ms
Anwendungen F
Klonalität Monoclonal
Klon GK1.5
Wirt Rat
Isotype IgG2b
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)


Swiss Prot Num:
Mouse CTL clone V4 cells
Isotype control SM19R
Anwendung Flow cytometry: 3 μg/ml.
Background CD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. In its extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) and other ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Concentration 0.5 mg/ml
General Readings
  1. Dialynas DP, Wilde DB, Marrack P, Pierres A, Wall KA, Havran W, et al. Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity. Immunol Rev. 1983;74:29-56. PubMed PMID: 6195085.
  2. Dialynas DP, Quan ZS, Wall KA, Pierres A, Quintáns J, Loken MR, et al. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol. 1983 Nov;131(5):2445-51. PubMed PMID: 6415170.
  3. Wilde DB, Marrack P, Kappler J, Dialynas DP, Fitch FW. Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J Immunol. 1983 Nov;131(5):2178-83. PubMed PMID: 6195255.
  4. Wu L, Antica M, Johnson GR, Scollay R, Shortman K. Developmental potential of the earliest precursor cells from the adult mouse thymus. J Exp Med. 1991 Dec 1;174(6):1617-27. PubMed PMID: 1683894. (Free PMC Article available)
  5. Godfrey DI, Kennedy J, Gately MK, Hakimi J, Hubbard BR, Zlotnik A. IL-12 influences intrathymic T cell development. J Immunol. 1994 Mar 15;152(6):2729-35. PubMed PMID: 7511624.
  6. Gavett SH, Chen X, Finkelman F, Wills-Karp M. Depletion of murine CD4+ T lymphocytes prevents antigen-induced airway hyperreactivity and pulmonary eosinophilia. Am J Respir Cell Mol Biol. 1994 Jun;10(6):587-93. PubMed PMID: 8003337.
  7. Zheng B, Han S, Kelsoe G. T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1. J Exp Med. 1996 Sep 1;184(3):1083-91. PubMed PMID: 9064325. (Free PMC Article available)
  8. Felix NJ, Donermeyer DL, Horvath S, Walters JJ, Gross ML, Suri A, et al. Alloreactive T cells respond specifically to multiple distinct peptide-MHC complexes. Nat Immunol. 2007 Apr;8(4):388-97. Epub 2007 Feb 25. PubMed PMID: 17322886.
  9. Hu M, Watson D, Zhang GY, Graf N, Wang YM, Sartor M, et al. Long-term cardiac allograft survival across an MHC mismatch after "pruning" of alloreactive CD4 T cells. J Immunol. 2008 May 15;180(10):6593-603. PubMed PMID: 18453578.
  10. Yi H, Zhen Y, Zeng C, Zhang L, Zhao Y. Depleting anti-CD4 monoclonal antibody (GK1.5) treatment: influence on regulatory CD4+CD25+Foxp3+ T cells in mice. Transplantation. 2008 Apr 27;85(8):1167-74. doi: 10.1097/TP.0b013e31816a1242. PubMed PMID: 18431238.
Storage Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Conjugated with R-Phycoerythrin under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Buffer System:
PBS, approx. pH 7.4
15 mM sodium azide
Liquid purified Ig fraction
Species Reactivity
Species reactivity (tested):
This antibody reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).
Gene ID 12504

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