AM26020PU-N CD62P / P-Selectin Antikörper

See related secondary antibodies

Search for all "CD62P / P-Selectin"

0.1 mg / 210,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Kurzübersicht

Mouse anti Human, Primate CD62P / P-Selectin AK4

AM26020PU-N

Produktbeschreibung für CD62P / P-Selectin

Mouse anti Human, Primate CD62P / P-Selectin AK4.
Presentation: Aff - Purified
Product is tested for Functional assay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Immunoprecipitation, Flow Cytometry.

Produktdaten von CD62P / P-Selectin

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 0.1 mg
Synonyme CD62 antigen-like family member P, GMP-140, GMRP, GRMP, Granule membrane protein 140, LECAM3, Leukocyte-endothelial cell adhesion molecule 3, PADGEM, SELP
Präsentation Aff - Purified
Reaktivität Hu, Prim
Anwendungen F, FN, ICC/IF, IP, WB
Klonalität Monoclonal
Klon AK4
Wirt Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datenblatt-Auszug

Immunogen
Swiss Prot Num:
P16109
Immunogen:
Human platelets
GeneID:
6403
Anwendung Flow cytometry: 1.5 µg/ml.
Immunoprecipitation.
Western blot.
Immunocytochemistry.
Functional application: Blocking.
Background CD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.
Concentration 1.0 mg/ml
General Readings
  1. Dunlop LC, Skinner MP, Bendall LJ, Favaloro EJ, Castaldi PA, Gorman JJ, et al. Characterization of GMP-140 (P-selectin) as a circulating plasma protein. J Exp Med. 1992 Apr 1;175(4):1147-50. PubMed PMID: 1372646. (Free PMC Article available)
  2. Holme PA, Müller F, Solum NO, Brosstad F, Frøland SS, Aukrust P. Enhanced activation of platelets with abnormal release of RANTES in human immunodeficiency virus type 1 infection. FASEB J. 1998 Jan;12(1):79-89. PubMed PMID: 9438413.
  3. Kowalska MA, Ratajczak J, Hoxie J, Brass LF, Gewirtz A, Poncz M, et al. Megakaryocyte precursors, megakaryocytes and platelets express the HIV co-receptor CXCR4 on their surface: determination of response to stromal-derived factor-1 by megakaryocytes and platelets. Br J Haematol. 1999 Feb;104(2):220-9. PubMed PMID: 10050701.
  4. Ludwig RJ, Schultz JE, Boehncke WH, Podda M, Tandi C, Krombach F, et al. Activated, not resting, platelets increase leukocyte rolling in murine skin utilizing a distinct set of adhesion molecules. J Invest Dermatol. 2004 Mar;122(3):830-6. PubMed PMID: 15086572.
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Purification:
Protein-A affinity chromatography
Buffer System:
Phosphate buffered saline (PBS)
Preservatives:
15 mM sodium azide, approx. pH 7.4
State:
Liquid Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human, Non-Human Primates
Specificity
Specificity:
This antibody recognizes CD62P (P-selectin), a 140 kD single chain type I transmembrane glycoprotein present in secretory alpha-granules in platelets, in Weibel-Palade bodies in endothelial cells and in megakaryocytes; it is relocated to the plasma membrane upon activation.

Accessory Products

Proteins and/or Positive Controls

Proteine zu CD62P / P-Selectin (13 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

CD62P / P-Selectin

CD62P / P-Selectin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / 748,00 €
  OriGene Technologies, Inc.

CD62P / P-Selectin

CD62P / P-Selectin Human Purified >95.0% as determined by SDS-PAGE.
Single band on Western Blot
E. coli
  OriGene Technologies GmbH

CD62P / P-Selectin

CD62P / P-Selectin Human Purified >95.0% as determined by SDS-PAGE.
Single band on Western Blot
E. coli
  OriGene Technologies GmbH

CD62P / P-Selectin

CD62P / P-Selectin Human Purified >95.0% as determined by SDS-PAGE.
Single band on Western Blot
E. coli
  OriGene Technologies GmbH

CD62P / P-Selectin

CD62P / P-Selectin Human Purified > 95.0% as determined by SDS-PAGE.  
Single band on Western Blot.
E. coli
  OriGene Technologies GmbH

CD62P / P-Selectin

CD62P / P-Selectin Human Purified > 95.0% as determined by SDS-PAGE.  
Single band on Western Blot.
E. coli
  OriGene Technologies GmbH

CD62P / P-Selectin

CD62P / P-Selectin Human Purified > 95.0% as determined by SDS-PAGE.  
Single band on Western Blot.
E. coli
  OriGene Technologies GmbH

CD62P / P-Selectin

CD62P / P-Selectin Human
  Abnova Taiwan Corp.

CD62P / P-Selectin

CD62P / P-Selectin Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD62P / P-Selectin

CD62P / P-Selectin Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD62P / P-Selectin

CD62P / P-Selectin Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD62P / P-Selectin

CD62P / P-Selectin Human Purified
  Novus Biologicals Inc.

CD62P / P-Selectin

CD62P / P-Selectin Human Purified
  Novus Biologicals Inc.

Positive controls for CD62P / P-Selectin (2 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

CD62P Lysate

Western Blot: P-Selectin Lysate [NBL1-15793] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for P-Selectin
  Novus Biologicals Inc.

SELP 293T Cell Transient Overexpression Lysate(Denatured)

SELP 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.
  • LinkedIn