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AF6110 Collagen type V Antikörper

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Mouse anti Human, Rabbit, Rat Collagen type V V-3C9

Produktbeschreibung für Collagen type V

Mouse anti Human, Rabbit, Rat Collagen type V V-3C9.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Frozen Sections, Western blot / Immunoblot, Paraffin Sections.

Produktdaten von Collagen type V

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 0.1 mg
Synonyme COL5A1
Präsentation Purified
Reaktivität Hu, Rb, Rt
Anwendungen C, E, P, WB
Klonalität Monoclonal
Klon V-3C9
Wirt Mouse
Isotype IgM
Molecular weight 180 kDa (Predicted)
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datenblatt-Auszug

Immunogen
Swiss Prot Num:
P20908
GeneID:
1289
Isotype control SM13P
Anwendung Western blot: 5 µg/ml.
Immunohistochemistry on Frozen and Paraffin Sections: 1-10 µg/ml. 
Heat induced antigen retrieval with citrat buffer, pH 6.2 using a pressure cooker was preformed. Sections were blocking using a commercially available casein solution. Signal was generated using a commercially available polymer HRP detection system and DAB
PLP fixation is required, antigen retrieval is HIER. Not recommended for traditional formalin fixation. Acetone fixation can also be used.
Background Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure.
Type V Collagen is a member of group I Collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V Collagen binds to DNA, heparan sulfate, Thrombospondin, Heparin, and Insulin. Collagen V is composed of several subunits as follows: trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Type V Collagen is closely related to type XI Collagen and it is possible that the Collagen chains of types V and XI constitute a single collagen type with tissue specific chain combinations. Mutations in this gene are associated with Ehlers Danlos syndrome, types I and II.
Concentration 0.5 mg/ml
General Readings
  1. Mayne R, Zettergren JG, Mayne PM, Bedwell NW. Isolation and partial characterization of basement membrane-like collagens from bovine thoracic aorta. Artery. 1980;7(4):262-80. PubMed PMID: 7213017.
  2. Niyibizi C, Fietzek PP, van der Rest M. Human placenta type V collagens. Evidence for the existence of an alpha 1(V) alpha 2(V) alpha 3(V) collagen molecule. J Biol Chem. 1984 Nov 25;259(22):14170-4. PubMed PMID: 6501291.
Storage Upon receipt, store (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein A
Buffer System:
0.1M Sodium Phosphate buffer, pH 7.0
Stabilizers:
0.5% BSA (Protease-free)
State:
Liquid purified Ig fraction
Purified
Species Reactivity
Species reactivity (tested):
Human, Rabbit and Rat.
Specificity
Specificity:
The antibody specifically reacts with hCL(V) (Human Collagen type V) and cross-reacts with Rabbit and Rat CL(V).
It recognizes the Collagen alpha-1(V) chain. 
Cellular Localization: Extracellular matrix.
Gene ID 1289

Accessory Products

Proteins and/or Positive Controls

Proteine zu Collagen type V (4 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

Collagen type V

Collagen type V Human > 98 % This product is chromatographically and Immunologically pure. Placenta
0.5 mg / 400,00 €
  OriGene Technologies GmbH

Collagen type V

Collagen type V Bovine Purified Prepared from Bovine Placenta and is chromatographically and immunologically pure Placenta
0.5 mg / 365,00 €
  OriGene Technologies GmbH

Collagen type V alpha 1 chain

Collagen type V Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Collagen type V alpha 1 chain

Collagen type V Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.
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