R1188 CREB1 (N-term) Antikörper

See related secondary antibodies

Search for all "CREB1"

0.1 ml / 515,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Kurzübersicht

Rabbit anti Human, Mouse, Rat CREB1

Produktbeschreibung für CREB1

Rabbit anti Human, Mouse, Rat CREB1.
Properties: (N-term)
Presentation: Liquid
Product is tested for Electrophoretic Mobility Shift Assay, Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Produktdaten von CREB1

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 0.1 ml
Synonyme CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1
Präsentation Liquid
Reaktivität Hu, Ms, Rt
Anwendungen E, EMSA, IP, WB
Klonalität Polyclonal
Wirt Rabbit
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datenblatt-Auszug

Immunogen
Swiss Prot Num:
P16220
Immunogen:
CREB-1 (p43) peptide corresponding to a region near the N-terminus of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
GeneID:
1385
Eigenschaften N-term
Anwendung Western Blot: 1/5,00-1/1,000. 
This antibody was assayed against a lysate of 1 x 106 Raji B cells by Immunoblot and found to be reactive against CREB-1 (p43), showing a 46 kDa band at a dilution of 1/500-1/1,000 followed by reaction with Peroxidase conjugated anti-Rabbit IgG (H&L) [Goat] (Cat#R1364HRP)
ELISA: 1/5,000-1/25,000.
Immunoprecipitation.
Gel Shift: This antibody was also tested in a Gel Supershift Assay and found to be reactive against CREB-1 (p43) using 0.5-1.0 µl per assay.
Background Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44 / 42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK 1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB appears to play an important role in learning and memory. CREB knock out mice show diminished learning ability.
Concentration 2.56 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Moreno, C.S., Beresford, G., Louis-Plence, P., Morris, A.C., and Boss, J.M. (1999) CREB regulates MHC class II expression in a CIITA dependent manner. Immunity 10: 143-151.
Storage Store the antibody (in aliquots) at -20°C.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: 6 month from despatch.
Format
Buffer System:
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
Preservatives:
0.01% (w/v) Sodium Azide
Stabilizers:
None
State:
Liquid (sterile filtered) Serum
Liquid
Specificity
Specificity:
This product was prepared from monospecific antiserum by delipidation and Immunoadsorption against an E.coli lysate immobilized on agarose beads.
This antibody may react non-specifically with other proteins. A partial cross-reactivity is observed against CREM-1 protein.
Species:
Human, Mouse and Rat.
Gene ID 1385
FocusOn and Reviews
Bewertungen:

Accessory Products

Proteins and/or Positive Controls

Proteine zu CREB1 (13 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

CREB1 (full length, N-term HIS tag, transcript variant A)

CREB1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / 205,00 €
  OriGene Technologies, Inc.

CREB1

CREB1 Human Purified > 95 % Greater than 95% as determined by SDS-PAGE. E. coli
  OriGene Technologies GmbH

CREB1

CREB1 Human Purified > 95 % Greater than 95% as determined by SDS-PAGE. E. coli
  OriGene Technologies GmbH

CREB1

CREB1 Human Purified > 95 % Greater than 95% as determined by SDS-PAGE. E. coli
  OriGene Technologies GmbH

CREB1

CREB1 Human
  Abnova Taiwan Corp.

CREB1

CREB1 Human in vitro transl.
  Abnova Taiwan Corp.

CREB1

CREB1 Human in vitro transl.
  Abnova Taiwan Corp.

CREB1

CREB1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CREB1

CREB1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CREB1

CREB1 Human Purified
  Abnova Taiwan Corp.

CREB1

CREB1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CREB1

CREB1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CREB1

CREB1 Human
  Abnova Taiwan Corp.

Positive controls for CREB1 (4 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

CREB1 293T Cell Transient Overexpression Lysate(Denatured)

CREB1 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

CREB1 HEK293 Cell Transient Overexpression Lysate (Non-Denatured)

CREB1 HEK293 Cell Transient Overexpression Lysate (Non-Denatured) Transient overexpression cell lysate was tested with Anti-CREB1 antibody (H00001385-M02) by Western Blots.
  Abnova Taiwan Corp.

CREB Lysate

Western Blot: CREB Lysate [NBL1-09461] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for CREB1
  Novus Biologicals Inc.

CREB Lysate

Western Blot: CREB Lysate [NBL1-09462] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for CREB1
  Novus Biologicals Inc.
  • LinkedIn