BM371 Desmoplakin (DP1+DP2) Antikörper

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50 µg / 320,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Kurzübersicht

Mouse anti Amphibian, Bovine, Canine, Chicken, Human, Mouse, Rat Desmoplakin DP2.15

BM371

Produktbeschreibung für Desmoplakin

Mouse anti Amphibian, Bovine, Canine, Chicken, Human, Mouse, Rat Desmoplakin DP2.15.
Properties: (DP1+DP2)
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Produktdaten von Desmoplakin

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 50 µg
Synonyme 250/210 kDa paraneoplastic pemphigus antigen, DP, DSP
Präsentation Purified
Reaktivität Amph, Bov, Can, Chk, Hu, Ms, Rt
Anwendungen C, ICC/IF, WB
Klonalität Monoclonal
Klon DP2.15
Wirt Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datenblatt-Auszug

Immunogen
Swiss Prot Num:
P15924
Immunogen:
Bovine Desmoplakin 1 and 2
GeneID:
1832
Eigenschaften DP1+DP2
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Anwendung Immunoblotting.
Immunofluorescence.
Immunohistochemistry on Frozen Sections:
Dilute 1/10 with PBS, pH 7.4 
Incubation Time: 1 h at RT.
Background Desmosomes are intercellular junctions that form tight links between adjacent cells. Desmoplakin is an obligate component of functional desmosomes that attaches intermediate filaments to desmosomal plaques. It is involved in the organization of desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains. The N-terminus of desmoplakin is essential for localisation to the desmosome and interaction with plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds to intermediate filaments. The central region of desmoplakin comprises a coiled-coil rod domain that mediates homodimerisation. There are two isoforms of desmoplakin - desmoplakin I, which is an obligate component of all desmosomes, and desmoplakin II, which is predominantly expressed in tissues and cells of stratified origin. Mutations in the gene encoding desmoplakin result in a number of cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus.
General Readings
  1. Cowin P, Kapprell HP, Franke WW. The complement of desmosomal plaque proteins in different cell types. J Cell Biol. 1985 Oct;101(4):1442-54. PubMed PMID: 2413044. (Free PMC Article available)
  2. Dockhorn-Dworniczak, B., Franke, W.W., Schröder, S., Czernobilsky, B., Gould, V.E. and Böcker, W.: Patterns of expression of cytoskeletal protein in human thyroid gland and thyroid carcinomas. Differentiation 35, 53-71 (1987).
  3. Franke WW, Moll R, Mueller H, Schmid E, Kuhn C, Krepler R, et al. Immunocytochemical identification of epithelium-derived human tumors with antibodies to desmosomal plaque proteins. Proc Natl Acad Sci U S A. 1983 Jan;80(2):543-7. PubMed PMID: 6340102. (Free PMC Article available)
  4. Koch PJ, Walsh MJ, Schmelz M, Goldschmidt MD, Zimbelmann R, Franke WW. Identification of desmoglein, a constitutive desmosomal glycoprotein, as a member of the cadherin family of cell adhesion molecules. Eur J Cell Biol. 1990 Oct;53(1):1-12. PubMed PMID: 1706270.
  5. Moll I, Moll R. Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas. Arch Dermatol Res. 1991;283(5):300-9. PubMed PMID: 1718227.
  6. Moll R, Cowin P, Kapprell HP, Franke WW. Desmosomal proteins: new markers for identification and classification of tumors. Lab Invest. 1986 Jan;54(1):4-25. PubMed PMID: 2417037.
  7. Owaribe K, Kartenbeck J, Stumpp S, Magin TM, Krieg T, Diaz LA, et al. The hemidesmosomal plaque. I. Characterization of a major constituent protein as a differentiation marker for certain forms of epithelia. Differentiation. 1990 Dec;45(3):207-20. PubMed PMID: 2090522.
  8. Owaribe K, Nishizawa Y, Franke WW. Isolation and characterization of hemidesmosomes from bovine corneal epithelial cells. Exp Cell Res. 1991 Feb;192(2):622-30. PubMed PMID: 1988297.
  9. Schmelz M, Franke WW. Complexus adhaerentes, a new group of desmoplakin-containing junctions in endothelial cells: the syndesmos connecting retothelial cells of lymph nodes. Eur J Cell Biol. 1993 Aug;61(2):274-89. PubMed PMID: 8223718.
  10. Hatzfeld M, Haffner C, Schulze K, Vinzens U. The function of plakophilin 1 in desmosome assembly and actin filament organization. J Cell Biol. 2000 Apr 3;149(1):209-22. PubMed PMID: 10747098. (Free PMC Article available, 12 images available)
  11. Bosch FX, Andl C, Abel U, Kartenbeck J. E-cadherin is a selective and strongly dominant prognostic factor in squamous cell carcinoma: a comparison of E-cadherin with desmosomal components. Int J Cancer. 2005 May 1;114(5):779-90. PubMed PMID: 15609307.
Storage

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Purification:
Affinity Chromatography on Protein A
Buffer System:
Final solution contains PBS, pH 7.4
Preservatives:
0.09% Sodium Azide
Stabilizers:
0.5% BSA
State:
Lyophilized purified Ig fraction
Purified
Reconstitution:
Restore in 1 ml distilled water
Species Reactivity
Species reactivity (tested):
Human, Bovine, Rat, Mouse and Chicken.
Specificity
Specificity:
This antibody reacts to Desmoplakin 1&2.
It shows distinct punctate membrane staining of different epithelia.
Tumors Specifically Detected: Primary and metastatic carcinoma and meningioma.
Polypeptides Reacting: Desmoplakin 1 and 2 (Mr 250 000 and 215 000).
Reactivity on Tested Cell Lines: Several Human carcinoma cell lines: MCF-7, A-431; Rat MHC1C1 cell line; Bovine cells: MDBK, BMGE.
Gene ID 1832

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