TA326414 Heat shock factor 1 / HSF1 Antikörper

Mouse monoclonal HSF1 Antibody

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Search for all "Heat shock factor 1 / HSF1"

0.1 mg / 330,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Kurzübersicht

Rat anti Bovine, Guinea Pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat Heat shock factor 1 / HSF1 10H8

Produktbeschreibung für Heat shock factor 1 / HSF1

Rat anti Bovine, Guinea Pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat Heat shock factor 1 / HSF1 10H8.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Electrophoretic Mobility Shift Assay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Produktdaten von Heat shock factor 1 / HSF1

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 0.1 mg
Synonyme HSF-1, HSTF1, Heat shock transcription factor 1
Präsentation Purified
Reaktivität Bov, GP, Hst, Hu, Mky, Ms, Rb, Rt
Anwendungen E, EMSA, ICC/IF, IP, WB
Klonalität Monoclonal
Klon 10H8
Wirt Rat
Isotype IgG1
Shipping to Europe, USA/Canada
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies, Inc.

Datenblatt-Auszug

Immunogen
Swiss Prot Num:
Q00613
Immunogen:
Purified recombinant HSF1 protein
GeneID:
3297
Isotype control SM25P, SM25PX
Anwendung Western blot (see ref. 1).
Immunoprecipitation.
Immunocytochemistry (see ref. 1).
ELISA (see ref. 1).
Gel Mobility Shift Assay (see ref. 1).
Background HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the Nterminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6).
There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).
Concentration 1.0 mg/ml
General Readings
  1. Cotto J.J., Fox S.G. and Morimoto R.I. (1997) J. Cell Science 110: 2925-2934.
  2. Morano K.A. and Thiele D.J. (1999). Gene Expression 7 (6): 271-82.
  3. Tanaka KI et al. (2007). JBC Papers Online Manuscript M704081200.
  4. Morimoto R. I. (1998) Genes Dev 12: 3788-3796.
  5. McMillan D. R., Xiao X., Shao L., Graves K., and Benjamin I. J. (1998) J Bio Chem 273: 7523-7528.
  6. Jolly C., Usson Y. and Morimoto R.I. (1999) Proc. Natl. Acad. Sci. USA 96 (12): 6769- 6774.
  7. Fiorenza M.T., Farkas T., Dissing M., Kolding D. and Zimarino V. (1995) Nucleic Acids Res. 23 (3):467-474.
  8. Goodson M.L., Park-Sarge O.K. and Sarge K.D. (1995) Mol. Cell. Biol. 15(10): 5288-5293.
  9. Rallu M., et al. (1997) Proc. Natl. Acad. Sci. USA 94(6): 2392-2397.
  10. Sarge K.D., et al. (1994) Biol. Reprod. 50(6): 1334-1343.
  11. Murphy S.P., Gorzowski J.J., Sarge K.D. and Phillips B. (1994) Mol. Cell. Biol. 14(8):5309-5317.
Storage Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
PBS, pH 7.4, 0.09% sodium azide in 50% glycerol
State:
Liquid purified Ig fraction
Purified
Species Reactivity
Species reactivity (tested):
Human, Mouse, Rat, Bovine, Guinea pig, Hamster, Monkey, Rabbit
Specificity
Specificity:
This antibody detects an ~85kDa protein in unstressed cell lysates, and an ~95kDa protein in heat shocked cell lysates, corresponding to the molecular mass of inactive and active forms of HSF1 on SDS-PAGE immunoblots.

Accessory Products

Proteins and/or Positive Controls

Proteine zu Heat shock factor 1 / HSF1 (8 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

Heat shock factor 1 / HSF1

Heat shock factor 1 / HSF1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / 748,00 €
  OriGene Technologies, Inc.

Heat shock factor 1 / HSF1

Heat shock factor 1 / HSF1 Human Purified > 75 % > 75% by SDS PAGE E. coli
0.5 mg / 1.070,00 €
  OriGene Technologies GmbH

Heat shock factor 1 / HSF1

Heat shock factor 1 / HSF1 Human Purified > 75 % E. coli
0.1 mg / 400,00 €
  OriGene Technologies GmbH

Heat shock factor 1 / HSF1

Heat shock factor 1 / HSF1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Heat shock factor 1 / HSF1

Heat shock factor 1 / HSF1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Heat shock factor 1 / HSF1

Heat shock factor 1 / HSF1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Heat shock factor 1 / HSF1

Heat shock factor 1 / HSF1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Heat shock factor 1 / HSF1

SDS-Page: HSF1 Protein [NBC1-18373] - HSF1, 59.4kDa (549aa) with a purity of 75% by SDS - PAGE Protein
  Novus Biologicals Inc.

Positive controls for Heat shock factor 1 / HSF1 (1 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

HSF1 overexpression lysate

HSF1 overexpression lysate
0.1 mg / 295,00 €
  OriGene Technologies, Inc.
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