TA319159 MIF Antikörper

Chicken polyclonal anti-MIF antibody

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0.5 mg / 571,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Kurzübersicht

Chicken anti Human MIF

Produktbeschreibung für MIF

Chicken anti Human MIF.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Produktdaten von MIF

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 0.5 mg
Synonyme GLIF, Glycosylation-inhibiting factor, MMIF, Macrophage migration inhibitory factor, Phenylpyruvate tautomerase
Präsentation Purified
Reaktivität Hu
Anwendungen E, WB
Klonalität Polyclonal
Wirt Chicken
Shipping to Europe, USA/Canada
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies, Inc.

Datenblatt-Auszug

Immunogen
Swiss Prot Num:
P14174
Immunogen:
This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to aa 2-32 of Human MIF conjugated to keyhole limpet hemocyanin (KLH). MIF is a proinflammatory cytokine that plays an important role in systemic inflammatory events.
AA Sequence:
P-M-F-I-V-N-T-N-V-P-R-A-S-V-P-D-G-F-L-S-E-L-T-Q-Q-L-A-Q-A-T-G
GeneID:
4282
Anwendung This IgY fraction antibody of anti-Human Macrophage
Migration Inhibitory Factor (MIF) has been tested for use in ELISA and Immunoblotting. Although not tested, this antibody may also be useful for Neutralization assays, Immunohistochemistry and Flow Cytometry. The antibody recognizes 12,300 MW mature human MIF. The MIF gene encodes
a protein of 115 aa. The initiating methionine is cleaved leaving a mature protein of 114 aa. Reactivity in other immunoassays is unknown.
Immunoblot: Using IRDYE800 conjugated Goat-a-Chicken IgG [H&L] MX10. A working dilution range of 1:500-1:1,000 is suggested for this application to detect human MIF from supernatants or lysates of 2 x 10e6 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E.coli LPS.
This product has been assayed by ELISA against human MIF using HRP Conjugated Goat-a-Chicken IgG [H&L] MX10 and ABTS as a substrate for 30 minutes at room temperature.
A working dilution range of 1:1,000-1:2,000 is suggested for this product. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody.
Background Cytokines play an important role in inflammation and immunity. Macrophage migration inhibitory factor (MIF) was one of the first cytokine activities described and is a proinflammatory cytokine that plays an important role in systemic inflammatory events. MIF's cytokine activity was initially described as a T cell-derived factor that inhibited the random migration of macrophages, hence its name. Since it was cloned and expressed in pure form, MIF's activities have been established to play many roles in development and various disease states. For example, MIF is released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counterregulates the immunosuppressive effects of steroids on immune cell activation and cytokine production. In in vitro experiments MIF is significantly upregulated by the stimulation of lipopolysaccharide (LPS) using 10 pg/ml to 10 ng/ml of LPS and reaches the maximum 12 h after the stimulation. MIF also prevents cleavage of Bax into an 18-kDa active fragment, and, consequently, reduces activation of the critical effector caspase 3, suggesting that MIF inhibits apoptosis pathways proximal to mitochondria activation and is therefore a survival factor. And, MIF is also known to exhibit enzymatic activities. A pathologic role for MIF has been described in many conditions including arthritis, asthma, and inflammatory bowel disease, and may also play a role in the control of cell growth in certain cancers. Consequently MIF is suggested to be a potential therapeutic target for human diseases.
Concentration 5.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Weiser WY, Temple PA, Witek-Giannotti JS, Remold HG, Clark SC, David JR. Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor. Proc Natl Acad Sci U S A. 1989 Oct;86(19):7522-6. PubMed PMID: 2552447. (Free PMC Article available)
  2. Bernhagen J, Calandra T, Mitchell RA, Martin SB, Tracey KJ, Voelter W, et al. MIF is a pituitary-derived cytokine that potentiates lethal endotoxaemia. Nature. 1993 Oct 21;365(6448):756-9. PubMed PMID: 8413654.
  3. Calandra T, Bernhagen J, Metz CN, Spiegel LA, Bacher M, Donnelly T, et al. MIF as a glucocorticoid-induced modulator of cytokine production. Nature. 1995 Sep 7;377(6544):68-71. PubMed PMID: 7659164.
  4. Mitchell R, Bacher M, Bernhagen J, Pushkarskaya T, Seldin MF, Bucala R. Cloning and characterization of the gene for mouse macrophage migration inhibitory factor (MIF). J Immunol. 1995 Apr 15;154(8):3863-70. PubMed PMID: 7706726.
  5. Rosengren E, Bucala R, Aman P, Jacobsson L, Odh G, Metz CN, et al. The immunoregulatory mediator macrophage migration inhibitory factor (MIF) catalyzes a tautomerization reaction. Mol Med. 1996 Jan;2(1):143-9. PubMed PMID: 8900542. (Free PMC Article available)
  6. Claret FX, Hibi M, Dhut S, Toda T, Karin M. A new group of conserved coactivators that increase the specificity of AP-1 transcription factors. Nature. 1996 Oct 3;383(6599):453-7. PubMed PMID: 8837781.
  7. Bucala R. Neuroimmunomodulation by macrophage migration inhibitory factor (MIF). Ann N Y Acad Sci. 1998 May 1;840:74-82. PubMed PMID: 9629239.
  8. Bernhagen J, Calandra T, Bucala R. Regulation of the immune response by macrophage migration inhibitory factor: biological and structural features. J Mol Med (Berl). 1998 Mar;76(3-4):151-61. PubMed PMID: 9535548.
  9. Kleemann R, Kapurniotu A, Frank RW, Gessner A, Mischke R, Flieger O, et al. Disulfide analysis reveals a role for macrophage migration inhibitory factor (MIF) as thiol-protein oxidoreductase. J Mol Biol. 1998 Jul 3;280(1):85-102. PubMed PMID: 9653033.
  10. Nishihira J, Koyama Y, Mizue Y. Identification of macrophage migration inhibitory factor (MIF) in human vascular endothelial cells and its induction by lipopolysaccharide. Cytokine. 1998 Mar;10(3):199-205. PubMed PMID: 9576065.
  11. Leech M, Metz C, Hall P, Hutchinson P, Gianis K, Smith M, et al. Macrophage migration inhibitory factor in rheumatoid arthritis: evidence of proinflammatory function and regulation by glucocorticoids. Arthritis Rheum. 1999 Aug;42(8):1601-8. PubMed PMID: 10446857.
  12. Kleemann R, Hausser A, Geiger G, Mischke R, Burger-Kentischer A, Flieger O, et al. Intracellular action of the cytokine MIF to modulate AP-1 activity and the cell cycle through Jab1. Nature. 2000 Nov 9;408(6809):211-6. PubMed PMID: 11089976.
  13. Onodera S, Kaneda K, Mizue Y, Koyama Y, Fujinaga M, Nishihira J. Macrophage migration inhibitory factor up-regulates expression of matrix metalloproteinases in synovial fibroblasts of rheumatoid arthritis. J Biol Chem. 2000 Jan 7;275(1):444-50. PubMed PMID: 10617637.
Storage Store vial at 2-8°C prior to restoration. Restore with 0.1 ml of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.
Avoid cycles of freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative.
State:
Lyophilized purified Ig fraction.
Purified
Reconstitution:
Restore with 0.1 ml of deionized water (or equivalent).
Specificity
Specificity:
This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum. The antibody is directed against the 12,300 MW human MIF protein and is useful in determining its presence in various assays. If neutralization experiments are performed for human MIF activity in bioassays, it is recommended to incubate the sample with a 1:500 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal chicken IgG is recommended. If FACS analysis experiments are performed for human MIF caution should be exhibited as the F( c) domain of the chicken IgG molecule may interact with cells non-specifically.
Endotoxin Content: < 10 pg/µl by LAL method.

Accessory Products

Proteins and/or Positive Controls

Proteine zu MIF (15 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

MIF

MIF Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / 438,00 €
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MIF

MIF Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
25 µg / 139,00 €
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MIF (1-115)

MIF Human Purified > 98 % pure by SDS PAGE E. coli
0.5 mg / 990,00 €
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MIF (1-115)

MIF Human Purified > 98 % pure by SDS PAGE E. coli
0.1 mg / 340,00 €
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MIF (1-115, His-tag)

MIF Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / 730,00 €
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MIF (1-115, His-tag)

MIF Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / 300,00 €
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MIF (1-115, His-tag)

MIF Human Purified > 95 % by SDS-PAGE E. coli
0.1 mg / 230,00 €
  OriGene Technologies GmbH

MIF (1-115, His-tag)

MIF Human Purified > 95 % by SDS-PAGE E. coli
0.5 mg / 540,00 €
  OriGene Technologies GmbH

MIF

MIF Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

MIF

MIF Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
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MIF

MIF Human
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MIF

MIF Human Purified > 95 % E. coli
5 µg / 190,00 €
  Neuromics Antibodies

MIF

MIF Human Purified > 95 % E. coli
25 µg / 290,00 €
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MIF

MIF Human Purified
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MIF

MIF Human Purified
  Novus Biologicals Inc.

Positive controls for MIF (1 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

MIF Lysate

Western Blot: MIF Lysate [NBL1-13108] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for MIF
  Novus Biologicals Inc.
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