DDX0420-HRPO-100 MIP3 alpha / CCL20 Antikörper

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0.1 mg / 490,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Kurzübersicht

Mouse anti Human MIP3 alpha / CCL20 206D9.05

DDX0420-HRPO-100

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  • DDX0420-HRPO-100
  • DDX0420-HRPO-100

Produktbeschreibung für MIP3 alpha / CCL20

Mouse anti Human MIP3 alpha / CCL20 206D9.05.
Presentation: HRP
Product is tested for ELISA (detection).

Produktdaten von MIP3 alpha / CCL20

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 0.1 mg
Synonyme Beta chemokine exodus-1, C-C motif chemokine 20, CC chemokine LARC, CCL20, DC marker, Dendritic Cell Marker, Exodus-1, LARC, Liver and activation-regulated chemokine, MIP-3 alpha, MIP3A, Macrophage inflammatory protein 3 alpha, SCYA20, Small-inducible cytokine A20
Präsentation HRP
Reaktivität Hu
Anwendungen E(detection)
Klonalität Monoclonal
Klon 206D9.05
Wirt Mouse
Isotype IgG1
Shipping to Germany, USA/Canada
PDF datasheet Datenblatt ansehen
Hersteller Dendritics SAS

Datenblatt-Auszug

Immunogen
Swiss Prot Num:
P78556
Immunogen:
Human recombinant MIP3α in eukaryotic cells.
Anwendung DDX0420P-50 DDX0420P-100 Purified: Blocking cell migration, ImmunoHistoChemistry frozen sections, ImmunoHistoChemistry paraffin sections, Western Blot.   
DDX0420HRPO-50 DDX0420HRPO-100 HRPO: ELISA detection (with 319F6.06 clone). 

Usage recommendation:
*This monoclonal antibody may be used between 5-20 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before using.
Background Macrophage inflammatory protein (MIP)-3alpha/ CCL20 is a CC-type chemokine mapped to chromosome 2 in humans. Langerhans cells (LCs) represent a unique population of DCs colonizing epithelium. MIP-3alpha plays a central role in LC precursor recruitment into the epithelium during inflammation. (a) Among DC populations, MIP-3alpha is the most potent chemokine inducing the selective migration of in vitrogenerated CD34(+) hematopoietic progenitor cell-derived LC precursors and skin LCs in accordance with the restricted MIP-3alpha receptor (CC chemokine receptor 6) expression by these cells. (b) MIP-3alpha is mainly produced by epithelial cells, and the migration of LC precursors induced by the supernatant of activated skin keratinocytes is blocked with an antibody against MIP-3alpha. (c) In vivo, MIP-3alpha is selectively produced at sites of inflammation as illustrated in tonsils and lesional psoriatic skin where MIP- 3alpha upregulation appear associated with an increase in LC turnover. (d) The secretion of MIP-3alpha is strongly upregulated by cells of epithelial origin after inflammatory stimuli (interleukin 1beta plus tumor necrosis factor alpha) or T cell signals.(Dieu-Nosjean et al, J;Exp.med, 2000 192: 705-18;Homey B. et al, 2000,J.Immunol.164:6621-32).
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Dieu-Nosjean MC, Massacrier C, Homey B, Vanbervliet B, Pin JJ, Vicari A, et al. Macrophage inflammatory protein 3alpha is expressed at inflamed epithelial surfaces and is the most potent chemokine known in attracting Langerhans cell precursors. J Exp Med. 2000 Sep 4;192(5):705-18. PubMed PMID: 10974036. (Free PMC Article available, 10 images available)
  2. Homey B, Dieu-Nosjean MC, Wiesenborn A, Massacrier C, Pin JJ, Oldham E, et al. Up-regulation of macrophage inflammatory protein-3 alpha/CCL20 and CC chemokine receptor 6 in psoriasis. J Immunol. 2000 Jun 15;164(12):6621-32. PubMed PMID: 10843722.
  3. Li Q, Estes JD, Schlievert PM, Duan L, Brosnahan AJ, Southern PJ, et al. Glycerol monolaurate prevents mucosal SIV transmission. Nature. 2009 Apr 23;458(7241):1034-8. doi: 10.1038/nature07831. Epub 2009 Mar 4. PubMed PMID: 19262509. (Free PMC Article available, 5 images available)
  4. Debeer S, Le Luduec JB, Kaiserlian D, Laurent P, Nicolas JF, Dubois B, et al. Comparative histology and immunohistochemistry of porcine versus human skin. Eur J Dermatol. 2013 Jul-Aug;23(4):456-66. doi: 10.1684/ejd.2013.2060. PubMed PMID: 24047577.
Storage Aliquot storage conditions -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Format
Label:
HRP
Purification:
QMA Hyper D ion exchange chromatography
Buffer System:
Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol.
Specificity
Specificity:
Human MIP-3α.

Accessory Products

Proteins and/or Positive Controls

Proteine zu MIP3 alpha / CCL20 (16 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

MIP3 alpha / CCL20 (transcript variant 1)

MIP3 alpha / CCL20 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
20 µg / 230,00 €
  OriGene Technologies, Inc.

MIP3 alpha / CCL20 (transcript variant 1)

MIP3 alpha / CCL20 Human > 98 %
Preparation: .
Purity Detail: >98%, as determined by Coomassie stained SDS-PAGE.
E. coli
10 µg / 195,00 €
  OriGene Technologies, Inc.

MIP3 alpha / CCL20

MIP3 alpha / CCL20 Human > 99 % E. coli
  OriGene Technologies GmbH

MIP3 alpha / CCL20

MIP3 alpha / CCL20 Human > 99 % E. coli
  OriGene Technologies GmbH

MIP3 alpha / CCL20

MIP3 alpha / CCL20 Mouse > 99 % E. coli
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MIP3 alpha / CCL20 Mouse > 99 % E. coli
  OriGene Technologies GmbH

MIP3 alpha / CCL20 (27-96, His-tag)

MIP3 alpha / CCL20 Human Purified > 85 % E. coli
0.25 mg / 1.070,00 €
  OriGene Technologies GmbH

MIP3 alpha / CCL20 (27-96, His-tag)

MIP3 alpha / CCL20 Human Purified > 85 % E. coli
50 µg / 400,00 €
  OriGene Technologies GmbH

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  Abnova Taiwan Corp.

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MIP3 alpha / CCL20 Human Purified
  Novus Biologicals Inc.

MIP3 alpha / CCL20

MIP3 alpha / CCL20 Human Purified
  Novus Biologicals Inc.

MIP3 alpha / CCL20

MIP3 alpha / CCL20 Human Purified
  Novus Biologicals Inc.

MIP3 alpha / CCL20

MIP3 alpha / CCL20 Human Purified
  Novus Biologicals Inc.

MIP3 alpha / CCL20

MIP3 alpha / CCL20 Human Purified
  Novus Biologicals Inc.

MIP3 alpha / CCL20

MIP3 alpha / CCL20 Human Purified
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Positive controls for MIP3 alpha / CCL20 (2 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

CCL20 overexpression lysate

CCL20 overexpression lysate
0.1 mg / 295,00 €
  OriGene Technologies, Inc.

Macrophage Inflammatory Protein 3 alpha Lysate

Western Blot: Macrophage Inflammatory Protein 3 alpha Lysate [NBL1-08850] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for CCL20
  Novus Biologicals Inc.
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