AF8410 MMP-14 / MT1-MMP Antikörper

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0.1 mg / 300,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Mouse anti Human, Mouse, Rabbit, Rat MMP-14 / MT1-MMP 113-5B7

AF8410

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Produktbeschreibung für MMP-14 / MT1-MMP

Mouse anti Human, Mouse, Rabbit, Rat MMP-14 / MT1-MMP 113-5B7.
Presentation: Purified
Product is tested for Frozen Sections, Paraffin Sections, Western blot / Immunoblot.

Produktdaten von MMP-14 / MT1-MMP

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 0.1 mg
Synonyme MMP-X1, MMP14, Matrix metalloproteinase-14, Membrane-type-1 matrix metalloproteinase
Präsentation Purified
Reaktivität Hu, Ms, Rb, Rt
Anwendungen C, P, WB
Klonalität Monoclonal
Klon 113-5B7
Wirt Mouse
Isotype IgG3
Molecular weight ~59-69 kDa
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datenblatt-Auszug

Immunogen
Swiss Prot Num:
P50281
Immunogen:
Oligopeptide of CDGNFDTVAMLRGEM (residue 319-333) on Human Membrane type 1 Matrix Metalloproteinase (hMT1-MMP).
GeneID:
4323
Isotype control AM03097PU-N
Anwendung Western Blot: 10 µg/ml.
Immunoprecipitation
(See Sato T. et al. 1994).
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections:
2-4 µg/ml. 
Heat induced antigen retrieval with citrat buffer, pH 6.2 using a pressure cooker was preformed. Sections were blocking using a commercially available casein solution. Signal was generated using a commercially available polymer HRP detection system and DAB.
Background Matrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components. Of the sixteen proteins reported to date, ten are normally found as soluble molecules. Several of the MMP proteins have been shown to be integral membrane proteins and have been named MT-MMPs for membrane bound MMP. The MT-MMP family is now known to contain at least three members, MT1-MMP, MT2-MMP and MT3-MMP, also known as MMP14, MMP15, and MMP16 respectively. While each of these proteins contain a C-terminal transmembrane domain allowing localization to the cell surface they are independent in expression. These proteins also differ from the other members of the MMP family in that they contain an 8 amino acid insert in the catalytic domain. The MT1-MMP protein is encoded by a 4.5 kb mRNA species giving rise to a protein with a molecular weight of 60-66 kDa by SDS-PAGE. MT1-MMP is responsible for cleaving progelatinase A (MMP-2, 72 kDa Type IV collagenase) and progelatinase B to their active forms. MT1-MMP itself requires an activation step that is the result of the activity of the membrane plasmin cascade. MT1-MMP functions by binding TIMP-2 and then the COOH terminal end of MMP-2 resulting in a 105 kDa trimer which effects the cleavage of pro-MMP-2 to the biologically active form. The order of the binding of pro-MMP-2 and TIMP-2 to MT1-MMP is critical as TIMP-2 will also inhibit the activity of MMP-2 when present in a soluble form.
Concentration 0.5 mg/ml
General Readings
  1. Sato, H. et al., Nature 370. 61-65, 1994.
  2. Yamada, T. et al., Acta Neuropathol., 90. 421-424, 1995.
  3. Sato, T. et al., J. Cell Sei., 110. 589-596, 1997.
  4. Kinoh, H. et al. J. Cell Sci., 109. 953-959, 1996.
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
0.1M Sodium Phosphate buffer, pH 7.0
Preservatives:
None
Stabilizers:
0.5% Protease-free BSA
State:
Liquid purified IgG fraction
Purified
Specificity
Specificity:
This monoclonal 113-5B7 antibody specifically reacts with Human MT1-MMP and cross-reacts with Mouse MT1-MMP. It slightly cross-reacts with Human MMP-3
Species:
Human, Mouse, Rat and Rabbit.
Gene ID 4323
FocusOn and Reviews
Bewertungen:

Accessory Products

Proteins and/or Positive Controls

Proteine zu MMP-14 / MT1-MMP (5 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

MMP-14 / MT1-MMP

MMP-14 / MT1-MMP Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / 680,00 €
  OriGene Technologies, Inc.

MMP-14 / MT1-MMP

MMP-14 / MT1-MMP Human SDS PAGE: >95%
  OriGene Technologies GmbH

MMP-14 / MT1-MMP

MMP-14 / MT1-MMP Human Purified
  Abnova Taiwan Corp.

MMP-14 / MT1-MMP

MMP-14 / MT1-MMP Human Purified
  Abnova Taiwan Corp.

MMP-14 / MT1-MMP

MMP-14 / MT1-MMP Human
  Abnova Taiwan Corp.

Positive controls for MMP-14 / MT1-MMP (2 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

MMP14 Lysate

Western Blot: MMP14 Lysate [NBL1-13153] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for MMP14
  Novus Biologicals Inc.

MMP14 overexpression lysate

MMP14 overexpression lysate
0.1 mg / 295,00 €
  OriGene Technologies, Inc.
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