AM05309PU-N Myogenin Antikörper

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0.1 mg / 560,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Kurzübersicht

Mouse anti Human, Mouse, Rat Myogenin F12B

Produktbeschreibung für Myogenin

Mouse anti Human, Mouse, Rat Myogenin F12B.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Electrophoretic Mobility Shift Assay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Produktdaten von Myogenin

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 0.1 mg
Synonyme BHLHC3, Class C basic helix-loop-helix protein 3, MYF4, MYOG, Myogenic factor 4
Präsentation Purified
Reaktivität Hu, Ms, Rt
Anwendungen E, EMSA, ICC/IF, IP, P, WB
Klonalität Monoclonal
Klon F12B
Wirt Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datenblatt-Auszug

Immunogen
Swiss Prot Num:
P20428
Immunogen:
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant protein containing rat myogenin amino acids 30-224 and mouse myeloma NS1 cells.
GeneID:
29148
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Anwendung ELISA.
Gel Supershift.
Immunohistochemistry on Formalin-fixed, Paraffin-embedded tissue sections (1-2 µg/ml).
Immunofluorescence.
Immunoprecipitation (2 µg/ml).
Western Blot (1 µg/ml).
Positive Control: Rhabdomyosarcoma and Wlim's tumors.
Background Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.
Concentration Lot specific
General Readings
  1. Wright WE, Dac-Korytko I, Farmer K. Monoclonal antimyogenin antibodies define epitopes outside the bHLH domain where binding interferes with protein-protein and protein-DNA interactions. Dev Genet. 1996;19(2):131-8. PubMed PMID: 8900045.
  2. Wright WE, Sassoon DA, Lin VK. Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD. Cell. 1989 Feb 24;56(4):607-17. PubMed PMID: 2537150.
  3. Auradé F, Pinset C, Chafey P, Gros F, Montarras D. Myf5, MyoD, myogenin and MRF4 myogenic derivatives of the embryonic mesenchymal cell line C3H10T1/2 exhibit the same adult muscle phenotype. Differentiation. 1994 Feb;55(3):185-92. PubMed PMID: 8187980.
Storage Store the antibody at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Buffer System:
PBS containing 0.08% Sodium Azide as preservative.
Purity:
Purified
State:
Liquid purified IgG fraction.
Purified
Specificity
Specificity:
Recognizes a protein of 34kDa, identified as myogenin. Its epitope localizes between aa 158-178 of myogenin.
This antibody can immunoprecipitate protein-DNA complex and is the antibody of choice for staining of formalin-fixed, paraffin-embedded tissues.
Species:
Human, Mouse and Rat.
Gene ID 29148

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