AM05309PU-N Myogenin Antikörper

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0.1 mg / 560,00 €
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Mouse anti Human, Mouse, Rat Myogenin F12B

Produktbeschreibung für Myogenin

Mouse anti Human, Mouse, Rat Myogenin F12B.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Electrophoretic Mobility Shift Assay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Produktdaten von Myogenin

Produkt-Kategorie Primärantikörper
Menge 0.1 mg
Synonyme BHLHC3, Class C basic helix-loop-helix protein 3, MYF4, MYOG, Myogenic factor 4
Präsentation Purified
Reaktivität Hu, Ms, Rt
Anwendungen E, EMSA, ICC/IF, IP, P, WB
Klonalität Monoclonal
Klon F12B
Wirt Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)


Swiss Prot Num:
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant protein containing rat myogenin amino acids 30-224 and mouse myeloma NS1 cells.
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Anwendung ELISA.
Gel Supershift.
Immunohistochemistry on Formalin-fixed, Paraffin-embedded tissue sections (1-2 µg/ml).
Immunoprecipitation (2 µg/ml).
Western Blot (1 µg/ml).
Positive Control: Rhabdomyosarcoma and Wlim's tumors.
Background Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.
Concentration Lot specific
General Readings
  1. Wright WE, Dac-Korytko I, Farmer K. Monoclonal antimyogenin antibodies define epitopes outside the bHLH domain where binding interferes with protein-protein and protein-DNA interactions. Dev Genet. 1996;19(2):131-8. PubMed PMID: 8900045.
  2. Wright WE, Sassoon DA, Lin VK. Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD. Cell. 1989 Feb 24;56(4):607-17. PubMed PMID: 2537150.
  3. Auradé F, Pinset C, Chafey P, Gros F, Montarras D. Myf5, MyoD, myogenin and MRF4 myogenic derivatives of the embryonic mesenchymal cell line C3H10T1/2 exhibit the same adult muscle phenotype. Differentiation. 1994 Feb;55(3):185-92. PubMed PMID: 8187980.
Storage Store the antibody at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Buffer System:
PBS containing 0.08% Sodium Azide as preservative.
Liquid purified IgG fraction.
Recognizes a protein of 34kDa, identified as myogenin. Its epitope localizes between aa 158-178 of myogenin.
This antibody can immunoprecipitate protein-DNA complex and is the antibody of choice for staining of formalin-fixed, paraffin-embedded tissues.
Human, Mouse and Rat.
Gene ID 29148

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