TA319254 NF-kB p105 / p50 Antikörper

Rabbit polyclonal anti-NFKB p50 antibody

See related secondary antibodies

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0.1 ml / 515,00 €
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Kurzübersicht

Rabbit anti Human NF-kB p105 / p50

Produktbeschreibung für NF-kB p105 / p50

Rabbit anti Human NF-kB p105 / p50.
Presentation: Serum
Product is tested for Enzyme Immunoassay, Electrophoretic Mobility Shift Assay, Immunoprecipitation, Western blot / Immunoblot.

Produktdaten von NF-kB p105 / p50

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 0.1 ml
Synonyme EBP-1, EBP1, KBF1, NF-kappa-B p50, NFKB1, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
Präsentation Serum
Reaktivität Hu
Anwendungen E, EMSA, IP, WB
Klonalität Polyclonal
Wirt Rabbit
Shipping to Europe, USA/Canada
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies, Inc.

Datenblatt-Auszug

Immunogen
Swiss Prot Num:
P19838
Immunogen:
Human NFkB p50 (NFKB1) peptide corresponding to a region near the N-terminus of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH)
GeneID:
4790
Anwendung Suitable for use in Immunoprecipitation, Immunoblotting (1/500-1/2,000), ELISA (1/5,000-1/25,000) and Supershift assays.
This product was assayed by Immunoblot and found to be reactive against Human NFkB p50 (NFKB1) at a dilution of 1/1000 followed by reaction with Peroxidase conjugated anti-Rabbit IgG [H&L] R1364HRP. Anti-Human NFkB p50 (NFKB1) is suitable for the detection by Immunoblot of Human NFkB p50 (NFKB1) and its precursor protein p105.
This product was also tested in a Gel Supershift assay and found to be reactive against p50:p50 homodimers and p:50:p65 heterodimers using 0.5 to 1.0 µl per assay.
Gel (Super) Shift Information:
In general, NFkB gel shift assays are assembled in 20 μl reactions containing 0.28 pmoles NFkB oligo in 10mM Tris (pH 7.6), 50 mM NaCl, 0.5 mM EDTA, 1.0 mM DTT, 10% glycerol. Some procedures specify the addition of 0.05% NP-40. When using purified protein, 250-300 ng should be sufficient to produce a gel shifted complex, while 10 ?g HeLa nuclear extract is utilized. The gel shift reactions are then incubated at room temperature for 30 minutes. The complexes are resolved on a Tris-Glycine acrylamide gels.
Loading dye containing bromophenol blue and xylene cyanol should be added to the negative control reaction only, as these dyes can increase the dissociation of the NFkB complexes. When using HeLa nuclear extract as the source of binding proteins, two sequence-specific gel-shifted complexes are expected, consisting of p50/p50 homodimers and p50/p65 heterodimers. For cells expressing p52, p50, and p65, as many as four sequence-specific gel-shifted complexes could be observed (p52/p52, p50/p50, p52/p65, p50/p65), and if high levels of p65 are present, the p65/p65 homodimer may also be weakly detected. The following reagents have been observed to enhance NFkB binding in vitro: millimolar amounts of GTP and ATP, spermine, spermidine, barium or calcium ions, and ?M amounts of Co+3(NH3)6.
Background NFκB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkB bound to IkB. NFkB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52(NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkB-alpha. IkB-alpha binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.
Concentration 80.0 mg/ml (by Refractometry)
Storage Aliquot contents and freeze at -20°C or below.
Avoid repeated freezing and thawing.
Dilute only prior to immediate use.
Shelf life: six months from despatch.
Format
Purification:
This product was prepared from monospecific antiserum by delipidation and defibrination.
State:
Liquid (sterile filtered) Serum containing 0.01% (w/v) Sodium Azide.
Serum
Specificity
Specificity:
Anti-NFkB p50 may react non-specifically with other proteins. Control peptide (#R1008PEP) will compete only with the specific reaction of antiserum with Human NFkB p50 (NFKB1).
Species:
Human. In immunoblot no reaction was observed against the analogous Mmouse protein.

Accessory Products

Proteins and/or Positive Controls

Proteine zu NF-kB p105 / p50 (5 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

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Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
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NF-kB p105 / p50 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
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NF-kB p105 / p50

NF-kB p105 / p50 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
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NF-kB p105 / p50

NF-kB p105 / p50 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
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Positive controls for NF-kB p105 / p50 (3 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

NF-kappaB-p105/p50 Lysate

Western Blot: NF-kappaB-p105/p50 Lysate [NBL1-13619] - Western Blot experiments. 
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