SM6006 SETD7 / SET9 (1-366) Antikörper

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0.1 ml / 330,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Kurzübersicht

Mouse anti Human SETD7 / SET9 s4E5

SM6006

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Produktbeschreibung für SETD7 / SET9

Mouse anti Human SETD7 / SET9 s4E5.
Properties: (1-366)
Presentation: Purified
Product is tested for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Enzyme Immunoassay, Western blot / Immunoblot, Paraffin Sections.

Produktdaten von SETD7 / SET9

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 0.1 ml
Synonyme H3-K4-HMTase SETD7, Histone H3-K4 methyltransferase SETD7, Histone-lysine N-methyltransferase SETD7, KIAA1717, KMT7, Lysine N-methyltransferase 7, SET domain-containing protein 7, SET7, SET7/9
Präsentation Purified
Reaktivität Hu
Anwendungen E, F, ICC/IF, P, WB
Klonalität Monoclonal
Klon s4E5
Wirt Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datenblatt-Auszug

Immunogen
Swiss Prot Num:
Q8WTS6
Immunogen:
Recombinant Human SET7/9 protein (1-366aa) purified from E. coli
GeneID:
80854
Eigenschaften 1-366
Isotype control AM03110PU-N, SM12P
Add. information Predicted Molecular Weight: 50 kDa.
Anwendung ELISA.
Western blot: 
1/500-1/2,000. 
Recommended Starting Dilution: 1/1,000.
Immunohistochemistry on Paraffin Sections: 5 µg/ml after heat induced antigen retrieval in pH 6.0 citrate buffer.
Immunocytochemistry / Immunoflourescence.
Flow cytometry.

Background Set7/9 is a histone methyltransferase (HMTase) that transfers methyl group to Lys4 of histone H3, in complex with S-adenosyl-L-methionine (AdoMet). The methylation of lysine residues of histones plays a critical role in the regulation of chromatin structure and gene expression.
Acetylation, phosphorylation and methylation of the amino-terminal tails of histone are thought to be involved in the regulation of chromatin structure and function. The enzymes identified in the methylation of specific lysine residue on histones belong to the SET family with just one exception. Set7/9, unlike most other SET proteins, is exclusively a mono-methylase.
Concentration 1.0 mg/ml
General Readings
  1.  Protein demethylation required for DNA methylation. Hotz HR, Peters AH.Nat Genet. 2009 Jan;41(1):10-1.
  2. Xiao B, Jing C, Wilson JR, Walker PA, Vasisht N, Kelly G, et al. Structure and catalytic mechanism of the human histone methyltransferase SET7/9. Nature. 2003 Feb 6;421(6923):652-6. Epub 2003 Jan 22. PubMed PMID: 12540855.
  3. Kwon T, Chang JH, Kwak E, Lee CW, Joachimiak A, Kim YC, et al. Mechanism of histone lysine methyl transfer revealed by the structure of SET7/9-AdoMet. EMBO J. 2003 Jan 15;22(2):292-303. PubMed PMID: 12514135. (Free PMC Article available, 5 images available)
  4. Nishioka K, Chuikov S, Sarma K, Erdjument-Bromage H, Allis CD, Tempst P, et al. Set9, a novel histone H3 methyltransferase that facilitates transcription by precluding histone tail modifications required for heterochromatin formation. Genes Dev. 2002 Feb 15;16(4):479-89. PubMed PMID: 11850410. (Free PMC Article available, 7 images available)
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein G
Buffer System:
PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
State:
Liquid purified IgG fraction
Purified
Specificity
Specificity:
The antibody recognizes Human SET7/9.
Other species not tested.
Gene ID 80854
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteine zu SETD7 / SET9 (9 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

SETD7 / SET9

SETD7 / SET9 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / 748,00 €
  OriGene Technologies, Inc.

SETD7 / SET9

SETD7 / SET9 Human Purified > 95 % by SDS-PAGE E. coli
0.1 mg / 250,00 €
  OriGene Technologies GmbH

SETD7 / SET9

SETD7 / SET9 Human Purified > 95 % by SDS-PAGE E. coli
0.5 mg / 600,00 €
  OriGene Technologies GmbH

SETD7 / SET9

SETD7 / SET9 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

SETD7 / SET9

SETD7 / SET9 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

SETD7 / SET9

SETD7 / SET9 Human
  Abnova Taiwan Corp.

SETD7 / SET9

SETD7 / SET9 Human
  Abnova Taiwan Corp.

SETD7 / SET9

SETD7 / SET9 Human
  Abnova Taiwan Corp.

SETD7 / SET9

SDS-Page: SETD7/9 Protein [NBC1-18444] - Set7/9, 40.7 kDa (366 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE Protein
  Novus Biologicals Inc.

Positive controls for SETD7 / SET9 (2 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

SETD7 Lysate

Western Blot: SETD7 Lysate [NBL1-15869] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for SETD7
  Novus Biologicals Inc.

SETD7 overexpression lysate

SETD7 overexpression lysate
0.1 mg / 295,00 €
  OriGene Technologies, Inc.
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