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GT15098-100 SOX2 Antikörper

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Kurzübersicht

Goat anti Human SOX2

Produktbeschreibung für SOX2

Goat anti Human SOX2.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Produktdaten von SOX2

Produkt-Kategorie Primärantikörper
Target-Kategorie
Menge 0.1 mg
Synonyme ANOP3, MCOPS3, SRY (sex determining region Y)-box 2, SRY-box 2, SRY-related HMG-box gene 2, Transcription factor SOX-2
Präsentation Aff - Purified
Reaktivität Hu
Anwendungen E, ICC/IF, WB
Klonalität Polyclonal
Wirt Goat
Shipping to Not USA/Canada
PDF datasheet Datenblatt ansehen
Hersteller Neuromics Antibodies

Datenblatt-Auszug

Immunogen
Swiss Prot Num:
P48431
Immunogen:
Recombinant human SOX2
GeneID:
6657
Anwendung Immunohistochemistry: 5-10 μg/mL.
Western Blot: 0.1 – 0.2 μg/mL. Tested for Western blotting using recombinant protein.
ELISA: 0.5 - 1.0 μg/mL.
Concentration 1 mg/ml
Protocols Application Notes
Immunocytochemistry - This antibody has been used at a concentration of 5 - 10 ug/mL to detect human SOX2. Cells were fixed with 4% paraformaldehyde in PBS at room temperature for 20 minutes, then blocked with PBS containing 0.1% Triton X-100, 1% BSA and 10% normal donkey serum at room temperature for 45 minutes. After blocking, sections were incubated with diluted primary antibody overnight at 4° C followed by Rhodamine Red coupled anti-goat IgG antibody or another appropriate secondary antibody at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA.
Western blot - This antibody can be used at 0.1 - 0.2 ug/mL with the appropriate secondary reagents to detect human SOX2. The detection limit for recombinant human SOX2 is approximately 2 ng/lane under non-reducing and reducing conditions.
Product Citations
Originator or purchased from resellers:
Jimena Andersenb. Study of Ascl1 function in the neurogenic lineage of the adult mouse hippocampus. Thesis 2015. 
http://discovery.ucl.ac.uk/1469969/1/PhD%20thesis_Jimena%20Andersen_Reduced.pdf
Format
Buffer System:
Solution in phosphate-buffered saline with 5% trehlose
State:
Liquid
Aff - Purified
Gene ID 6657

Accessory Products

Proteins and/or Positive Controls

Proteine zu SOX2 (16 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

SOX2

SOX2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / 748,00 €
  OriGene Technologies, Inc.

SOX2

SOX2 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
25 µg / 230,00 €
  OriGene Technologies, Inc.

SOX2

SOX2 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
25 µg / 230,00 €
  OriGene Technologies, Inc.

SOX2

SOX2 Human > 95 % pure by SDS-PAGE and HPLC analyses. E. coli
  OriGene Technologies GmbH

SOX2

SOX2 Human > 95 % pure by SDS-PAGE and HPLC analyses. E. coli
  OriGene Technologies GmbH

SOX2 (TAT peptide tagged)

SOX2 Human > 95 % pure by SDS-PAGE and HPLC analyses. E. coli
  OriGene Technologies GmbH

SOX2 (TAT peptide tagged)

SOX2 Human > 95 % pure by SDS-PAGE and HPLC analyses. E. coli
  OriGene Technologies GmbH

SOX2 (1-200, His-tag)

SOX2 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / 720,00 €
  OriGene Technologies GmbH

SOX2 (1-200, His-tag)

SOX2 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / 290,00 €
  OriGene Technologies GmbH

SOX2

SOX2 Human
  Abnova Taiwan Corp.

SOX2

SOX2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

SOX2

SOX2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

SOX2

SOX2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

SOX2

SOX2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

SOX2

SOX2 Human
  Abnova Taiwan Corp.

SOX2

SDS-Page: SOX2 Protein [NBP1-41144] - SOX2, 23.1kDa (208aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE
  Novus Biologicals Inc.

Positive controls for SOX2 (2 Produkte)

Katalog-Nr. Spezies Präs. Reinheit   Source  

SOX2 Lysate

Western Blot: SOX2 Lysate [NBL1-16348] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for SOX2
  Novus Biologicals Inc.

SOX2 overexpression lysate

SOX2 overexpression lysate
0.1 mg / 295,00 €
  OriGene Technologies, Inc.
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