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CL077F T Cell Receptor (TCR) V beta 4 Antikörper

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Kurzübersicht

Rat anti Mouse T Cell Receptor (TCR) V beta 4 CTVB4

Produktbeschreibung für T Cell Receptor (TCR) V beta 4

Rat anti Mouse T Cell Receptor (TCR) V beta 4 CTVB4.
Presentation: FITC
Product is tested for Flow Cytometry.

Produktdaten von T Cell Receptor (TCR) V beta 4

Produkt-Kategorie Primärantikörper
Menge 0.1 mg
Synonyme TCR V beta-4, TCR Vb4
Präsentation FITC
Reaktivität Ms
Anwendungen F
Klonalität Monoclonal
Klon CTVB4
Wirt Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datenblatt-Auszug

Anwendung Flow Cytometry.
Concentration 0.1 mg/ml
Protocols FLOW CYTOMETRY ANALYSIS:

Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test).
4. To each tube, add ~1.0 µg* of this Ab per 10e6 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most flurochromes are light sensitive.)
7. Wash 2 times at 4°C.
8. Resuspend the cell pellet in 50 µl ice cold media B.
9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls).

Results - Tissue Distribution by Flow Cytometry Analysis:
Mouse Strain: C3H.SW
Cell Concentration: 1x10e6 cells per tests
Antibody Concentration Used: 1.0 µg/10e6 cells
Isotypic Control: FITC Rat IgG2a
General Readings
  1. Tomonari, K., lovering, E. and Spencer, S. 1990 Correlation between the Vb4+ CD8+ T cell population and the H-2d haplotype. Immunogenetics 31:333-339.
  2. Padula, S.J., Lingenheld, E.G., Stabach, P.R., Chou, C.J., Kono, D.H. and Clark, R.B. 1991 Identificaion of encephalitogenic Vb4-bearing T cells in SJL mice-further evidence for the V region disease hyopothesis? J. Immunol. 146:879-883.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
This product is photosensitive and should protected from light.
Shelf life: one year from despatch.
Format
Label:
FITC
Buffer System:
PBS, 0.09% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
State:
Liquid purified Ig
Specificity
Specificity:
This antigen monoclonal antibody reats with TCR Vb4 bearing T cells. TCR Vb4 is expressed in most known mouse strains.
Species:
Mouse.

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