R1253B Mouse IgG (H+L chain) Antikörper

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2 mg / 230,00 €
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R1253B

Produktbeschreibung für Mouse IgG

Properties: (H+L chain)
Presentation: Biotin
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Produktdaten von Mouse IgG

Produkt-Kategorie Sekundärantikörper
Target-Kategorie
Menge 2 mg
Synonyme Mouse Immunoglobulin G
Präsentation Biotin
Anwendungen C, E, WB
Klonalität Polyclonal
Wirt Rabbit
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datenblatt-Auszug

Immunogen
Immunogen:
Mouse IgG whole molecule.
Anwendung Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/300,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Concentration 2.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990. (Conjugation)
Storage Store secondary antibody at 4° C prior to reconstitution.  
Centrifuge product if not completely clear after standing at room temperature. 
This product is stable for several weeks at 4° C as an undiluted liquid. 
Dilute only prior to immediate use. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing!
Format
Label:
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin
Molar radio:
10-20 BAC molecules per Rabbit IgG molecule
Purification:
Immunoaffinity Chromatography
Buffer System:
0.01M Sodium Phosphate, 0.14M Sodium Chloride, pH 7.4
Preservatives:
0.01% (w/v) Sodium Azide
Stabilizers:
10 mg/ml BSA (IgG and Protease free)
Purity:
and
State:
Lyohilized purified Ig fraction
Reconstitution:
Reconstitute  with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
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