R1253F Mouse IgG (H+L chain) Antikörper

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2 mg / 200,00 €
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R1253F

Produktbeschreibung für Mouse IgG

Properties: (H+L chain)
Presentation: FITC
Product is tested for Immunocytochemistry/Immunofluorescence, Enzyme Immunoassay.

Produktdaten von Mouse IgG

Produkt-Kategorie Sekundärantikörper
Target-Kategorie
Menge 2 mg
Synonyme Mouse Immunoglobulin G
Präsentation FITC
Anwendungen E, ICC/IF
Klonalität Polyclonal
Wirt Rabbit
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datenblatt-Auszug

Immunogen
Immunogen:
Mouse IgG whole molecule.
Anwendung Suitable for ELISA, Immunofluorescence Microscopy.
Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1000-1/5000.
Concentration 2.0 mg/ml (by UV absorbance at 280 nm)
Product Citations
Purchased from Acris:
  1. Koshkin V, Ailles LE, Liu G, Krylov SN. Metabolic suppression of a drug resistant subpopulation in cancer spheroid cells. J Cell Biochem. 2015 Jun 6. doi: 10.1002/jcb.25247. PubMed PMID: 26054050.
General Readings
  1. The and Feltkamp, Immunology 18; 865, 1970. (Conjugation)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.

Format
Label:
Fluorescein isothiocyanate (Molecular Weight 390 daltons).
Conjugation Chemistry: Isothiocyanate
FITC
Absorption emission:
495 nm / 528 nm
Molar radio:
3.1 moles FITC per mole of Rabbit IgG
Purification:
Immunoaffinity Chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservatives:
0.01% (w/v) Sodium Azide
Stabilizers:
10 mg/ml BSA (Ig and Protease free)
State:
Lyophilized purified IgG fraction
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
Species:
Mouse.
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