R1386HRP Porcine IgG F(ab)2 specific Antikörper

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2 mg / 305,00 €
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R1386HRP

Produktbeschreibung für Porcine IgG

Properties: F(ab)2 specific
Presentation: HRP

Produktdaten von Porcine IgG

Produkt-Kategorie Sekundärantikörper
Target-Kategorie
Menge 2 mg
Synonyme Pig IgG, Pig Immunoglobulin G
Präsentation HRP
Klonalität Polyclonal
Wirt Rabbit
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datenblatt-Auszug

Immunogen
Immunogen:
Swine IgG F(ab')2 fragment.
Anwendung Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Swine IgG F(ab')2 in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
General Readings
  1. Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
Storage Store vial at 4°C prior to restoration. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.
Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 4°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid cycles of freezing and thawing.
Shelf life: One year from despatch.
Format
Label:
Horseradish Peroxidase
HRP
Purification:
Immunoaffinity chromatography.
Buffer System:
0.01 M Sodium Phosphate, 0.14M Sodium Chloride, pH 7.4 with 0.01% (w/v) Gentamicin sulfate as preservative and 10 mg/ml Bovine Serum Albumin (BSA; IgG and Protease free) as stabilizer.
State:
Lyophilized purified Ig fraction.
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Swine IgG F(ab')2 and Swine Serum.
No reaction was seen against Swine IgG F(c).
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