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NB120-6024 Rabbit IgG Fc F(ab)2 Fragment Antikörper

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NB120-6024

Produktbeschreibung für Rabbit IgG Fc F(ab)2 Fragment

Presentation: AP
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Produktdaten von Rabbit IgG Fc F(ab)2 Fragment

Produkt-Kategorie Sekundärantikörper
Menge 0.25 mg
Synonyme Rabbit Immunoglobulin G
Präsentation AP
Reaktivität Rb
Anwendungen E, P, WB
Klonalität Polyclonal
Wirt Goat
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet Datenblatt ansehen
Hersteller Novus Biologicals Inc.

Datenblatt-Auszug

Immunogen
Immunogen:
Rabbit IgG F(ab')2 fragment
Add. information Dot: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/600 - 1/2800. IHC: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/600 to 1/2800 of the reconstitution concentration is suggested for this product.Suitable for other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Anwendung Dot Blot, ELISA, immunohistochemistry, Western Blot, ELISA, Immunohistochemistry, Western and Dot Blotting This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. Suitable for other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. , ELISA 1:300-1:15000, immunohistochemistry 1:200-1:1000, Western Blot 1:500-1:2500, Dot Blot
Concentration 1 mg/ml
General Readings General / background references:Avrameas S & Ternynck T Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration. Immunochemistry 8:1175-9 (1971). PubMed PMID: 5150003
Storage Store at 4C. Do not freeze.
Format
Label:
Alkaline Phosphatase
AP
Purification:
Immunogen affinity purified
Buffer System:
0.01% Sodium Azide
Purity:
Immunogen affinity purified
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(ab')2 ????
Species:
Rabbit
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