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R1458C2 Rabbit IgG (H+L chain, adsorbed) Antikörper

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R1458C2

Produktbeschreibung für Rabbit IgG

Properties: (H+L chain, adsorbed)
Presentation: Cy2

Produktdaten von Rabbit IgG

Produkt-Kategorie Sekundärantikörper
Target-Kategorie
Menge 1 mg
Synonyme Rabbit Immunoglobulin G
Präsentation Cy2
Klonalität Polyclonal
Wirt Goat
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datenblatt-Auszug

Immunogen
Immunogen:
Rabbit IgG, whole molecule
Anwendung This product is designed for Immunofluorescence Microscopy,
fluorescence based plate assays (FLISA) and fluorescent Western blotting.
This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000
Immunofluorescence: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings Conjugation: Modified from GE Healthcare Bio-Sciences Corp., Piscataway, NJ.
Storage Store vial at 2-8°C prior to restoration. For extended storage mix with glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
(Cyanine 2-OSu) (MW. 897 daltons)
Cy2
Absorption emission:
489 nm / 505 nm
Molar radio:
2.8 moles Cy2 per mole of Goat IgG
Purification:
Immunoaffinity Chromatography.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
State:
Lyophilized purified Ig fraction.
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
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