R1371HRP Rat IgG (H+L chain) Antikörper

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2 mg / 230,00 €
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R1371HRP

Produktbeschreibung für Rat IgG

Properties: (H+L chain)
Presentation: HRP
Product is tested for Frozen Sections, Western blot / Immunoblot, Enzyme Immunoassay.

Produktdaten von Rat IgG

Produkt-Kategorie Sekundärantikörper
Target-Kategorie
Menge 2 mg
Synonyme Rat Immunoglobulin G
Präsentation HRP
Anwendungen C, E, WB
Klonalität Polyclonal
Wirt Rabbit
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datenblatt-Auszug

Immunogen
Immunogen:
Rat IgG whole molecule
Anwendung Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidas antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/170,000
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500- 1/2,500.
Caution Do NOT add Sodium Azide!
Concentration 2.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981. (Conjugation)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term. 
Centrifuge product if not completely clear after standing at RT.   
Dilute only prior to immediate use. 
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Horseradish Peroxidase
HRP
Purification:
Immunoaffinity Chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Buffer System:
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
Preservatives:
0.01% (w/v) Gentamicin Sulfate
Stabilizers:
10 mg/ml BSA (IgG and Protease free)
State:
Lyophilized purified Ig fraction
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Rat IgG and Rat Serum.
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