AP05260CP-N SIAH1 Control Peptide

Search for all "SIAH1"

50 µg / 230,00 €
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Kurzübersicht

anti SIAH1

AP05260CP-N

Produktbeschreibung für SIAH1

anti SIAH1.
Properties: Control Peptide
Presentation: Purified

Produktdaten von SIAH1

Produkt-Kategorie Control Peptides
Target-Kategorie
Menge 50 µg
Synonyme E3 ubiquitin-protein ligase SIAH1, HUMSIAH, Seven in absentia homolog 1, Siah-1, Siah-1a
Präsentation Purified
Klonalität Polyclonal
Shipping to Worldwide
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datenblatt-Auszug

Immunogen
Swiss Prot Num:
Q8IUQ4
GeneID:
6477
Anwendung Incubate antibody neat with at least a 50 fold stoichiometric excess of blocking peptide at 37°C for 20 minutes (molecular weights of peptide and antibody are ~2.5 kDa and ~160 kDa, respectively). Antibody can then be diluted to a concentration suitable for Western blot.
Example: 10 µl or 10 µg of rabbit anti-SIAH1 is added to 10 µg of blocking peptide for a total volume of 20 µl. The mixture is allowed to incubate for 20 minutes at 37°C prior to dilution in suitable buffer (for Western blot, etc.).
Background Seven in absentia homolog 1 (SIAH-1) is a member of the RING-finger-containing E3 ubiquitin ligases. Alpha-synuclein and synphilin-1 are substrates of SIAH-1 , both proteins are involved in the development of Parkinson's disease (PD). Mutations in Parkin, another E3 ubiquitin ligase which ubiquinates synphilin-1 and glycosylated alpha-synuclein, have been defined as a major cause of autosomal recessive PD. The role of SIAH-1 in PD is highlighted by the fact that SIAH-1 is a component of the Lewy bodies and plays a role in apoptosis caused by nitric oxide (NO) induced oxidative stress. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) a classic glycolytic enzyme, and multi-functional protein. GAPDH plays role as a mediator for cell death. GAPDH translocates to the nucleus under a variety of stressors/conditions, most of which are associated with oxidative stress. Sequential steps lead to nuclear translocation of GAPDH during cell death; 1] a catalytic cysteine in GAPDH (C150 in rat GAPDH) is S-nitrosylated by nitric oxide (NO) which is generated from inducible nitric oxide synthase (iNOS) and/or neuronal NOS (nNOS); 2] the modified GAPDH becomes capable of binding with Siah1, an E3 ubiquitin ligase, and stabilizes it;3] the GAPDH-Siah protein complex translocates to the nucleus, dependent on Siah1's nuclear localization signal, and degrades Siah1's substrates in the nucleus, which results in cytotoxicity. GAPDH may be genetically associated with late-onset Alzheimer's disease. (-)-deprenyl, which has been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and has neuroprotective actions. The molecular action of this protection is as yet not clear.
Concentration Lot specific
Storage Store at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
Phosphate buffered saline containing 0.08% sodium azide
Purity:
Purified
State:
Liquid purified peptide
Purified
Specificity
Specificity:
Control peptide for antibody AP05260PU-N only.
Gene ID 6477

Accessory Products

  • LinkedIn