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EA101018 CA 72-4 CLIA Kit

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EA101018

Produktdaten von CA 72-4 CLIA Kit

Produkt-Kategorie ELISA Kits
Menge 1 kit
Synonyme CA 72-4
Reaktivität Hu
Shipping to Europe, USA/Canada
PDF datasheet Datenblatt ansehen
Hersteller OriGene Technologies, Inc.

Datenblatt-Auszug

Add. information
Interference:
No significant interference observed with available related molecules.
Anwendung
Specificity:
Natural and recombinant human CA 72-4
Assay Type:
Chemiluminescence Immunoassay (CLIA)
Signal:
Colorimetric
Sample Type:
Cell culture supernatant, serum, plasma
Sample Volume:
25 ul
Curve Range:
0 - 100 U/mL
Background The CA 72-4 CLIA is a chemiluminescence immunoassay (CLIA), based on the sandwich principle. The microtiter wells are coated with a monoclonal [mouse] antibody directed towards a unique antigenic site on the CA 72-4 molecule. An aliquot of donor sample containing endogenous CA 72-4 is incubated in the coated well with enzyme conjugate, which is an anti- CA 72-4 -antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of CA 72-4 in the sample. After addition of the substrate solution, the intensity of emitted light is proportional to the concentration of CA 72-4 in the donor sample.
Assaytime 3 hours
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