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CPK2220-50rxns hsa-mir-504 Real-Time RT-PCR Detection and U6 Calibration Kit

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CPK2220-50rxns

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Produktdaten von hsa-mir-504 Real-Time RT-PCR Detection and U6 Calibration Kit

Produkt-Kategorie PCR Kits
Menge 50 rxns
Reaktivität Hu
Shipping to Europe only
PDF datasheet Datenblatt ansehen
Hersteller Cohesion Biosciences Ltd.

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Anwendung Real-time RT-PCR
The Real-Time RT-PCR detection and U6 calibration Kit contains the whole reagent including the stem-loop RT primer & specific PCR primer set for microRNA quantificaion and U6 primer set as housekeeping gene for calibration. It provides a sensitive and specific method of real-time PCR for relative quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set.
Description: Detect mature miRNA hsa-mir-504 expression normalized by U6 snRNA.
PCR Method: 2-Step qRT-PCR
Detection Range: Seven orders of magnitude
Sensitivity: 10 copies of a standard mimics and as little as 25 pg of total RNA
Sample Types: miRNA, Total RNA
Platform: ABI 7500 System/ViiA™ 7 System/StepOne™/StepOnePlus™/7900HT System; BioRad IQ5.0/CFX Connect; Stratagen MX3000p/4000p; Roche LightCycler 480
Components: 50 rxns: It contains all reagents required to perform 50 cDNA reactions and 50 PCR reactions.100 rxns: It contains all reagents required to perform 100 cDNA reactions and 100 PCR reactions.200 rxns: It contains all reagents required to perform 200 cDNA reactions and 200 PCR reactions.
Storage Shipped at 4°C. Store at -20°C for 6 months.
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