14-3-3 protein sigma / SFN antibody

Principal name

14-3-3 protein sigma / SFN antibody

Alternative names for 14-3-3 protein sigma / SFN antibody

Stratifin, HME1, Epithelial cell marker protein 1

SwissProt ID

O70456 (Mouse), O77642 (Sheep), P31947 (Human), Q0VC36 (Bovin)

Gene ID

2810 (SFN), 443032, 55948 (Sfn)

Available reactivities

Hu (Human), Bov (Bovine), Chk (Chicken), Ms (Mouse), Rb (Rabbit), Rt (Rat), Sh (Sheep), Can (Canine), Por (Porcine), Ze (Zebrafish), Ce (C. elegans), Eq (Equine), GP (Guinea Pig), Amph (Amphibian), Op (Opossum)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Flow Cytometry (F)

Background of 14-3-3 protein sigma / SFN antibody

It is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein.
14-3-3 sigma is expressed mostly in epithelial cells and has been associated with cancers including breast, nasopharyngeal, endometrial adenocarcinoma, prostate, epithelial ovarian, colorectal, adenoid cystic, and papillary. 14-3-3 sigma binds c-fos activating the MAPK pathway. Sequestration of Bax by 14-3-3 sigma inhibits apoptosis whereas sequestration of 14-3-3 sigma by CARP-1 induces it. Mitosis is regulated by 14-3-3 sigma through binding keratin filaments and sequestering cyclin B1 and cdc2. p73-dependent induction of 14-3-3 sigma regulates chemo sensitivity in breast cancers with p53 mutations. In reaction to DNA damage and along with RACK1, 14-3-3 sigma targets, DeltaNp63alpha for proteasome degradation. 14-3-3 also negatively regulates both the glucocorticoid receptor and the activity of PKB/Akt which stabilizes p27 Kip1.

General readings

1. Leffers, H., Madsen, P., Rasmussen, H. H., Honore, B., Andersen, A. H., Walbum, E.,
Vandekerckhove, J., Celis, J. E.Molecular cloning and expression of the transformation sensitive
epithelial marker stratifin: a member of a protein family that has been involved in the protein kinase C
signalling pathway.J. Molec. Biol. 231: 982-998, 1993.
2. Urano, T., Saito, T., Tsukui, T., Fujita, M., Hosoi, T., Muramatsu, M., Ouchi, Y., Inoue, S. Efp targets
14-3-3-sigma for proteolysis and promotes breast tumour growth.Nature 417: 871-875, 2002.
3. Wilker, E. W., van Vugt, M. A. T. M., Artim, S. A., Huang, P. H., Petersen, C. P., Reinhardt, H. C., Feng,
Y., Sharp, P. A., Sonenberg, N., White, F. M., Yaffe, M. B.14-3-3-sigma controls mitotic translation to
facilitate cytokinesis.Nature 446: 329-332, 2007.

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