ACOT4 antibody
Principal name
ACOT4 antibody
Alternative names for ACOT4 antibody
PTE2B, PTE-2b, PTEIB, Acyl-CoA thioesterase 4, Acyl-coenzyme A thioesterase 4, Peroxisomal acyl coenzyme A thioester hydrolase Ib, Peroxisomal long-chain acyl-CoA thioesterase Ib
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of ACOT4 antibody
- Kit Component:
- - KN300734G1, Acot4 gRNA vector 1 in pCas-Guide vector
- KN300734G2, Acot4 gRNA vector 2 in pCas-Guide vector
- KN300734D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunohistochemical analysis of ACOT4 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of ACOT4 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of ACOT4 expression in RT4 (A); A549 (B) whole cell lysates.
Western blot analysis of extracts from HeLa cells and COLO cells, using ACOT4 antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of MCF-7 cells, using ACOT4 antibody.The picture on the right is treated with the synthesized peptide.
Host: Rabbit; Target Name: ACOT4; Sample Tissue: Lymph Node Tumor lysates; Antibody Dilution: 1.0 ug/ml
Primärantikörper
| Katalog-Nr. | Wirt | Iso. | Klon | Präs. | Reakt. | Anwendungen | |
|---|---|---|---|---|---|---|---|
| TA316066 | ACOT4 Antikörper |
||||||
| Rabbit polyclonal anti-ACOT4 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, ICC/IF, WB |
0.1 ml /
335,00 €
|
|
| OriGene Technologies, Inc. | |||||||
 +1 weiteres Bild |
|||||||
| TA344353 | ACOT4 Antikörper |
||||||
| Rabbit Polyclonal Anti-ACOT4 Antibody - middle region | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt | WB |
50 µg /
360,00 €
|
|
| OriGene Technologies, Inc. | |||||||
| C14272-1 | ACOT4 Antikörper |
||||||
 |
Rabbit | Aff - Purified | Hu | E, ICC/IF, WB |
50 µg /
230,00 €
|
||
| Assay Biotech | |||||||
| +1 weiteres Bild |
|||||||
| C14272-2 | ACOT4 Antikörper |
||||||
|
Rabbit | Aff - Purified | Hu | E, ICC/IF, WB |
0.1 mg /
330,00 €
|
||
| Assay Biotech | |||||||
| +1 weiteres Bild |
|||||||
CRISPR
| Katalog-Nr. | Spezies | Donor Vector | |
|---|---|---|---|
| KN300734 | Acot4 - mouse gene knockout kit via CRISPR |
||
 |
Mouse | GFP-Puro |
1 kit /
1.290,00 €
|
| OriGene Technologies, Inc. | |||
| KN300734BN | Acot4 - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
1.290,00 €
|
| OriGene Technologies, Inc. | |||
| KN300734LP | Acot4 - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
1.290,00 €
|
| OriGene Technologies, Inc. | |||
| KN300734RB | Acot4 - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
1.290,00 €
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Katalog-Nr. | Spezies | Präs. | Reinheit | Source | |
|---|---|---|---|---|---|
| TP310550 | ACOT4 |
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| Recombinant protein of human acyl-CoA thioesterase 4 (ACOT4) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
680,00 €
|
|
| OriGene Technologies, Inc. | |||||
Lysate
| Katalog-Nr. | ||
|---|---|---|
| LY407636 | ACOT4 overexpression lysate |
|
|
0.1 mg /
295,00 €
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Katalog-Nr. | ||
|---|---|---|
| RC210550L2V | Lenti ORF particles, ACOT4 (mGFP-tagged) - Human acyl-CoA thioesterase 4 (ACOT4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
1.082,00 €
|
|
| OriGene Technologies, Inc. | ||
| RC210550L1V | Lenti ORF particles, ACOT4 (Myc-DDK tagged) - Human acyl-CoA thioesterase 4 (ACOT4) , 200 uL, >10^7 TU/mL |
|
|
200 µl /
969,00 €
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Katalog-Nr. | ||
|---|---|---|
| MR218697L2V | Lenti ORF particles, Acot4 (GFP-tagged) - Mouse acyl-CoA thioesterase 4 (Acot4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
907,00 €
|
|
| OriGene Technologies, Inc. | ||
| MR218697L1V | Lenti ORF particles, Acot4 (Myc-DDK-tagged) - Mouse acyl-CoA thioesterase 4 (Acot4), 200 uL, >10^7 TU/mL |
|
|
200 µl /
804,00 €
|
|
| OriGene Technologies, Inc. | ||
