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UBE2L3 / UBCH7 antibody

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Principal name

UBE2L3 / UBCH7 antibody

Alternative names for UBE2L3 / UBCH7 antibody

Ubiquitin-protein ligase L3, Ubiquitin carrier protein L3, E2-F1, L-UBC, UBCE7, Ubiquitin-conjugating enzyme E2 L3

SwissProt ID

P68036 (Human), Q3MHP1 (Bovin)

Gene ID

7332 (UBE2L3)

Ncbi ID

9606, NP_003338.1, NP_003338, 5874

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Dros (Drosophila)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of UBE2L3 / UBCH7 antibody

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2L3 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro.This gene encodes a protein that has been identified as a component of NuRD, a nucleosome remodeling deacetylase complex identified in the nucleus of human cells. It shows a very broad expression pattern and is strongly expressed in many tissues. It may represent one member of a small gene family that encode different but related proteins involved either directly or indirectly in transcriptional regulation. Their indirect effects on transcriptional regulation may include chromatin remodeling. It is closely related to another member of this family, a protein that has been correlated with the metastatic potential of certain carcinomas. These two proteins are so closely related that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. One of the proteins known to be a target protein for this gene product is p53. Deacteylation of p53 is correlated with a loss of growth inhibition in transformed cells supporting a connection between these gene family members and metastasis.

General readings

Nuber U, Schwarz S, Kaiser P, Schneider R, Scheffner M. Cloning of human ubiquitin-conjugating enzymes UbcH6 and UbcH7 (E2-F1) and characterization of their interaction with E6-AP and RSP5. J Biol Chem. 1996 Feb 2;271(5):2795-800. PMID: 8576257

H00007332-M01 - UBE2L3 / UBCH7
Immunofluorescence of monoclonal antibody to UBE2L3 on HeLa cell. [antibody concentration 10 ug/ml]
H00007332-M01 - UBE2L3 / UBCH7
UBE2L3 monoclonal antibody (M01), clone 3B7 Western Blot analysis of UBE2L3 expression in K-562 ( Cat # L009V1 ).
PAB3893 - UBE2L3 / UBCH7
Formalin-fixed and paraffin-embedded human cancer tissue reacted with UBE2L3 polyclonal antibody ( Cat # PAB3893 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
PAB3893 - UBE2L3 / UBCH7
The UBE2L3 polyclonal antibody ( Cat # PAB3893 ) is used in Western blot to detect UBE2L3 in HL-60 cell lysate.
PAB6308 - UBE2L3 / UBCH7
UBE2L3 polyclonal antibody ( Cat # PAB6308 ) staining ( 0.5 µg/ml ) of HeLa lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
ARP43025_T100 - UBE2L3 / UBCH7
Human Intestine; UBE2L3 antibody - C-terminal region (ARP43025_T100) in Human Intestine cells using Immunohistochemistry
ARP43025_T100 - UBE2L3 / UBCH7
Human Jurkat; WB Suggested Anti-UBE2L3 Antibody Titration: 1.25ug/ml. Positive Control: Jurkat cell lysate. There is BioGPS gene expression data showing that UBE2L3 is expressed in Jurkat.; UBE2L3 antibody - C-terminal region (ARP43025_T100) in Human Jurkat cells using Western Blot
ARP43026_P050 - UBE2L3 / UBCH7
Human Prostate; Immunohistochemistry with Human Prostate lysate tissue at an antibody concentration of 5.0ug/ml using anti-UBE2L3 antibody (ARP43026_P050); UBE2L3 antibody - C-terminal region (ARP43026_P050) in Human Prostate cells using Immunohistochemistry
ARP43026_P050 - UBE2L3 / UBCH7
Human Jurkat; WB Suggested Anti-UBE2L3 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate. There is BioGPS gene expression data showing that UBE2L3 is expressed in Jurkat.; UBE2L3 antibody - C-terminal region (ARP43026_P050) in Human Jurkat cells using Western Blot
AP11976PU-N - UBE2L3 / UBCH7
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AP11976PU-N - UBE2L3 / UBCH7
Western blot analysis of anti-UBE2L3 Antibody (N-term) (Cat.#AP11976PU-N) to detect UBE2L3 in HL-60 cell lysate.
ARP43023_P050 - UBE2L3 / UBCH7
Human Testis; Testis; UBE2L3 antibody - middle region (ARP43023_P050) in Human Testis cells using Immunohistochemistry
ARP43023_P050 - UBE2L3 / UBCH7
Transfected 293T; WB Suggested Anti-UBE2L3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Transfected 293T; UBE2L3 antibody - middle region (ARP43023_P050) in Transfected 293T cells using Western Blot
TP308780 - UBE2L3 / UBCH7
TA302697 - UBE2L3 / UBCH7
TA302697 staining (0.5µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
TA308758 - UBE2L3 / UBCH7
UBE2L3 antibody detects UBE2L3 protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. 15 % SDS-PAGE. UBE2L3 antibody (TA308758) dilution: 1:1000
TA308758 - UBE2L3 / UBCH7
Sample (30 ug of whole cell lysate). A: Hela. B: Hep G2 . 12% SDS PAGE. TA308758 diluted at 1:1000.
TA308758 - UBE2L3 / UBCH7
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using UBE2L3(TA308758) antibody at 1:100 dilution.
TA308758 - UBE2L3 / UBCH7
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using UBE2L3(TA308758) antibody at 1:200 dilution.
TA308758 - UBE2L3 / UBCH7
Immunoprecipitation of UBE2L3 protein from HeLa whole cell extracts using 5 ug of UBE2L3 antibody (TA308758). Western blot analysis was performed using UBE2L3 antibody (TA308758). An anti-rabbit IgG was used as a secondary reagent.
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  2. Zitierungen (1)
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Primärantikörper

Katalog-Nr. Wirt Iso. Klon Präs. Reakt. Anwendungen  

UBE2L3 / UBCH7 Antikörper
  Rabbit Polyclonal antibody to UBE2L3 (ubiquitin-conjugating enzyme E2L 3)  
UBE2L3 antibody detects UBE2L3 protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. 15 % SDS-PAGE. UBE2L3 antibody (TA308758) dilution: 1:1000 Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / 416,00 €
  OriGene Technologies, Inc.
+4 weitere Bilder

Highly validated antibody!

UBE2L3 / UBCH7 (C-term) Antikörper
  Goat Polyclonal Antibody against UBE2L3  
TA302697 staining (0.5µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Por, Rt E, WB
0.1 mg / 360,00 €
  OriGene Technologies, Inc.

UBE2L3 / UBCH7 (N-term) Antikörper
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / 370,00 €
  OriGene Technologies GmbH
+1 weiteres Bild

Proteins & Growth Factors

Katalog-Nr. Spezies Präs. Reinheit Source  

UBE2L3 / UBCH7 (transcript variant 1)
  Recombinant protein of human ubiquitin-conjugating enzyme E2L 3 (UBE2L3), transcript variant 1  
UBE2L3 / UBCH7 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / 680,00 €
  OriGene Technologies, Inc.
 

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Produkttyp
  1. Primary Antibody (3)
  2. Protein | Growth Factor (1)
Klonalität
  1. Polyclonal (3)
Target
  1. UBE2L3 / UBCH7 (4)
Eigenschaften
  1. C-term (1)
  2. N-term (1)
Reaktivität
  1. Human (Hu) (3)
  2. Mouse (Ms) (2)
  3. Bovine (Bov) (1)
  4. Porcine (Por) (1)
  5. Rat (Rt) (1)
Anwendungen
  1. Western blot / Immunoblot (WB) (3)
  2. Paraffin Sections (P) (2)
  3. Enzyme Immunoassay (E) (1)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Präsentation
  1. Aff - Purified (2)
  2. Purified (1)
Wirt
  1. Rabbit (2)
  2. Goat (1)
Bilder
  1. 1 image (4)
  2. 3+ images (1)
  3. 5+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Immunofluorescence (IF) (1)
  3. IHC (1)
  4. Immunoprecipitation (IP) (1)
  5. Paraffin Sections (P) (1)
Spezies (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Hersteller
  1. OriGene Technologies (4)
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