WT1 / Wilms tumor protein antibody

Principal name

WT1 / Wilms tumor protein antibody

Alternative names for WT1 / Wilms tumor protein antibody

WT33

SwissProt ID

O62651 (Pig), P19544 (Human)

Gene ID

7490 (WT1)

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat), Chk (Chicken), Ze (Zebrafish), African clawed frog, Bov (Bovine), Eq (Equine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Frozen Sections (C)

Selected product citations ‐ of AP15857PU-M

1. Yates LL, Papakrivopoulou J, Long DA, Goggolidou P, Connolly JO, Woolf AS, et al. The planar cell polarity gene Vangl2 is required for mammalian kidney-branching morphogenesis and glomerular maturation. Hum Mol Genet. 2010 Dec 1;19(23):4663-76. doi: 10.1093/hmg/ddq397. Epub 2010 Sep 14. PubMed PMID: 20843830. 
2. Vasilopoulou, E;Kolatsi-Joannou, M;Lindenmeyer, MT;White, KE;Robson, MG;Cohen, CD;Sebire, NJ;Riley, PR;Winyard, PJ;Long, DA;2016Loss of endogenous thymosin β4 accelerates glomerular disease. Kidney Int. PubMed PMID: 27575556

Background of WT1 / Wilms tumor protein antibody

WT1 is a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm\'s tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms.This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm\'s tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.

General readings

Ruteshouser,E.C., (2008) Genes Chromosomes Cancer 47 (6), 461-470

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Primärantikörper

Katalog-Nr. Wirt Iso. Klon Präs. Reakt. Anwendungen  

WT1 / Wilms tumor protein Antikörper

  WT1 Rabbit Polyclonal Antibody  
Western blot analysis of kidney cell lysate using WT1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / 325,00 €
  OriGene Technologies, Inc.

WT1 / Wilms tumor protein Antikörper

  Mouse Monoclonal Antibody against Wilms Tumor 1 (6F-H2)  
Western Blot- Wilms Tumor 1 Antibody (6F-H2) detects WT1 in 293 cell lysate Mouse IgG1 6F-H2 Purified Hu P, WB
0.1 ml / 325,00 €
  OriGene Technologies, Inc.

WT1 / Wilms tumor protein Antikörper

  Goat Polyclonal Antibody against Wilms tumor 1 / WT1  
TA302633 (1µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / 325,00 €
  OriGene Technologies, Inc.

WT1 / Wilms tumor protein Antikörper

  Rabbit polyclonal antibody to WT1 (Wilms tumor 1)  
Wilms Tumor 1 antibody detects Wilms Tumor 1 protein at nucleus and cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Wilms Tumor 1 antibody (TA308577) dilution: 1:500. Rabbit IgG Aff - Purified Hu, Rt ICC/IF, P, WB
0.1 ml / 325,00 €
  OriGene Technologies, Inc.

WT1 / Wilms tumor protein Antikörper

  Mouse Monoclonal WT1 Antibody  
Immunohistochemistry staining of Paraffin Mesothelioma tissue by WT1 antibody (dilution: 1:100 - 1:500; visualization of staining: Nuclear) Mouse IgG1 6F-H2 Purified Hu P
1 ml / 450,00 €
  OriGene Technologies, Inc.

WT1 / Wilms tumor protein Antikörper

  Rabbit Polyclonal anti-WT1 Antibody  
Host: Rabbit; Target Name: WT1; Sample Tissue: Esophagus Tumor lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Hu WB
50 µg / 325,00 €
  OriGene Technologies, Inc.

WT1 / Wilms tumor protein Antikörper

  Rabbit Polyclonal Anti-WT1 Antibody  
WB Suggested Anti-WT1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Hela cell lysate Rabbit IgG Purified Hu WB
50 µg / 325,00 €
  OriGene Technologies, Inc.

WT1 / Wilms tumor protein Antikörper

  Rabbit Polyclonal Anti-WT1 Antibody  
WB Suggested Anti-WT1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: HT1080 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt P, WB
50 µg / 325,00 €
  OriGene Technologies, Inc.

WT1 / Wilms tumor protein Antikörper

  Rabbit Polyclonal anti-Wilm's Tumor (WT) Antibody  
Human Wilms Tumor stained with anti-Wilm's Tumor (WT) antibody. Rabbit Purified Hu P
0.1 ml / 325,00 €
  OriGene Technologies, Inc.

WT1 / Wilms tumor protein Antikörper

Formalin-Fixed, Paraffin-Embedded Human Wilms tumor stained with WT1 antibody Cat.-No. AM00403SU-L using peroxidase conjugate and DAB chromogen. Note the nuclear staining of tumor cells. Mouse IgG1 6F-H2 Supernatant Hu P
1 ml / 360,00 €
  Acris Antibodies GmbH

WT1 / Wilms tumor protein (349-439) Antikörper

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 1E9 Purified Hu E, P, WB
50 µg / 530,00 €
  Acris Antibodies GmbH

WT1 / Wilms tumor protein (Center) Antikörper

WT1 Antibody (Center E361) (AP54572PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WT1 Antibody (Center E361) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu ICC/IF, P, WB
0.4 ml / 370,00 €
  Acris Antibodies GmbH

CRISPR

Katalog-Nr. Spezies Donor Vector  

WT1 - human gene knockout kit via CRISPR

WT1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / 1.200,00 €
  OriGene Technologies, Inc.

WT1 - human gene knockout kit via CRISPR

WT1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / 1.290,00 €
  OriGene Technologies, Inc.

WT1 - human gene knockout kit via CRISPR

WT1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / 1.290,00 €
  OriGene Technologies, Inc.

WT1 - human gene knockout kit via CRISPR

WT1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / 1.290,00 €
  OriGene Technologies, Inc.

Proteins & Growth Factors

Katalog-Nr. Spezies Präs. Reinheit Source  

WT1 / Wilms tumor protein (full length, N-term HIS tag, transcript variant B)

  Purified recombinant protein of Human Wilms tumor 1 (WT1), transcript variant B, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
WT1 / Wilms tumor protein Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / 199,00 €
  OriGene Technologies, Inc.

WT1 / Wilms tumor protein (full length, N-term HIS tag, transcript variant A)

  Purified recombinant protein of Human Wilms tumor 1 (WT1), transcript variant A, full length, with N-terminal HIS tag, expressed in E. coli, 50ug  
WT1 / Wilms tumor protein Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / 199,00 €
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Katalog-Nr.    

Lenti ORF particles, WT1 (mGFP-tagged) - Human Wilms tumor 1 (WT1), transcript variant A, 200 uL, >10^7 TU/mL

Lenti ORF particles, WT1 (mGFP-tagged) - Human Wilms tumor 1 (WT1), transcript variant A, 200 uL, >10^7 TU/mL  
200 µl / 1.120,00 €
  OriGene Technologies, Inc.

Lenti ORF particles, WT1 (mGFP-tagged) - Human Wilms tumor 1 (WT1), transcript variant B, 200 uL, >10^7 TU/mL

Lenti ORF particles, WT1 (mGFP-tagged) - Human Wilms tumor 1 (WT1), transcript variant B, 200 uL, >10^7 TU/mL  
200 µl / 1.140,00 €
  OriGene Technologies, Inc.

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